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A single gene all3940 (Dps) overexpression in Anabaena sp. PCC 7120 confers multiple abiotic stress tolerance via proteomic alterations

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Abstract

DNA-binding proteins (Dps) induced during starvation play an important role in gene regulation and maintaining homeostasis in bacteria. The nitrogen-fixing cyanobacterium, Anabaena PCC7120, has four genes annotated as coding for Dps; however, the information on their physiological roles is limiting. One of the genes coding for Dps, ‘all3940’ was found to be induced under different abiotic stresses in Anabaena and upon overexpression enhanced the tolerance of Anabaena to a multitude of stresses, which included salinity, heat, heavy metals, pesticide, and nutrient starvation. On the other hand, mutation in the gene resulted in decreased growth of Anabaena. The modulation in the levels of All3940 in Anabaena, achieved either by overexpression of the protein or mutation of the gene, resulted in changes in the proteome, which correlated well with the physiological changes observed. Proteins required for varied physiological activities, such as photosynthesis, carbon-metabolism, oxidative stress alleviation, exhibited change in protein profile upon modulation of All3940 levels in Anabaena. This suggested a direct or an indirect effect of All3940 on the expression of the above stress-responsive proteins, thereby enhancing tolerance in Anabaena PCC7120. Thus, All3940, though categorized as a Dps, is possibly a general stress protein having a global role in regulating tolerance to multitude of stresses in Anabaena.

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Acknowledgments

L. C. Rai is thankful to DAE-BRNS, Mumbai, for the financial support in the form of project and DST New Delhi for J C Bose National Fellowship. OPN and NK are thankful to ICAR and CSIR for senior research fellowships, respectively.

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Authors and Affiliations

  1. Molecular Biology Section, Center of Advanced Study in Botany, Banaras Hindu University, Varanasi, 221 005, India

    Om Prakash Narayan, Nidhi Kumari & Lal Chand Rai

  2. Gujarat State Biotechnology Mission, Department of Science and Technology, Government of Gujarat, GandhiNagar, Gujarat, India

    Poonam Bhargava

  3. Molecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400 085, India

    Hema Rajaram

  4. Plant Biotechnology, Rajiv Gandhi South Campus, Banaras Hindu University, Barkachha, Mirzapur, 231301, India

    Om Prakash Narayan

  5. Department of Biological Sciences, Indian Institute of Science Education and Research, Sector-81, Mohali, 140 306, India

    Nidhi Kumari

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  1. Om Prakash Narayan
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  2. Nidhi Kumari
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  3. Poonam Bhargava
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  4. Hema Rajaram
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Correspondence to Lal Chand Rai.

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Narayan, O.P., Kumari, N., Bhargava, P. et al. A single gene all3940 (Dps) overexpression in Anabaena sp. PCC 7120 confers multiple abiotic stress tolerance via proteomic alterations. Funct Integr Genomics 16, 67–78 (2016). https://doi.org/10.1007/s10142-015-0467-7

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  • DOI: https://doi.org/10.1007/s10142-015-0467-7

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