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The Lesion Simulating Disease (LSD) gene family as a variable in soybean response to Phakopsora pachyrhizi infection and dehydration

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Abstract

The Lesion Simulating Disease (LSD) genes encode a family of zinc finger proteins that are reported to play an important role in the hypersensitive response and programmed cell death (PCD) that are caused by biotic and abiotic stresses. In the present study, 117 putative LSD family members were identified in Viridiplantae. Genes with one, two, or three conserved LSD domains were identified. Proteins with three LSD domains were highly represented in the species analyzed and were present in basal organisms. Proteins with two LSD domains were identified only in the Embryophyte clade, and proteins possessing one LSD domain were highly represented in grass species. Expression analyses of Glycine max LSD (GmLSD) genes were performed by real-time quantitative polymerase chain reaction. The results indicated that GmLSD genes are not ubiquitously expressed in soybean organs and that their expression patterns are instead organ-dependent. The expression of the majority of GmLSD genes is modulated in soybean during Phakopsora pachyrhizi infection. In addition, the expression of some GmLSD genes is modulated in plants under dehydration stress. These results suggest the involvement of GmLSD genes in the response of soybean to both biotic and abiotic stresses.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Genética e Biologia Molecular, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil

    Caroline Cabreira, Alexandro Cagliari, Lauro Bücker-Neto, Beatriz Wiebke-Strohm, Loreta B. de Freitas, Márcia M. A. N. Margis-Pinheiro & Maria H. Bodanese-Zanettini

  2. Empresa Brasileira de Pesquisa Agropecuária-Embrapa Soja, Londrina, PR, Brazil

    Francismar C. Marcelino-Guimarães & Alexandre L. Nepomuceno

  3. Departamento de Genética, Universidade Federal do Rio Grande do Sul-UFRGS, P. O. Box 15053, 91501-970, Porto Alegre, RS, Brazil

    Maria H. Bodanese-Zanettini

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  1. Caroline Cabreira
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  2. Alexandro Cagliari
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  3. Lauro Bücker-Neto
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Correspondence to Maria H. Bodanese-Zanettini.

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This work was supported by grants from the GENOSOJA/CNPq and BIOTECSUR II/MCT.

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Fig. S1

Relative expression levels of the GmLSD genes in each different organ of the Conquista Brazilian soybean cultivar. The values are the means of three biological replicates with four technical replicates each. The means that are labeled identically (with a letter) do not differ significantly (Tukey comparison test, p < 0.05). ACTII, CYP2 and metalloprotease reference genes were used as internal controls to normalize for the amount of mRNA present in each sample. In each organ, the gene with the lowest transcript level was used to normalize the transcript levels of the other genes. To allow a better comparison of groups of genes that exhibited large differences in their expression levels, some graphs of fold-change are shown in log2 scale, and this is indicated at the y axis. ND: no detected (PDF 40 kb)

Table S1

Summary of the GmLSD gene RT-qPCR primer sequences (PDF 51 kb)

Table S2

Species, acronym, locus and EST identified for the 117 LSD genes (PDF 149 kb)

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Cabreira, C., Cagliari, A., Bücker-Neto, L. et al. The Lesion Simulating Disease (LSD) gene family as a variable in soybean response to Phakopsora pachyrhizi infection and dehydration. Funct Integr Genomics 13, 323–338 (2013). https://doi.org/10.1007/s10142-013-0326-3

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