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Esophageal cancer alters the expression of nuclear pore complex binding protein Hsc70 and eIF5A-1

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Abstract

Nuclear pore complex (NPC) is the only corridor for macromolecules exchange between nucleus and cytoplasm. NPC and its components, nucleoporins, play important role in the diverse physiological processes including macromolecule exchange, chromosome segregation, apoptosis and gene expression. Recent reports also suggest involvement of nucleoporins in carcinogenesis. Applying proteomics, we analyzed expression pattern of the NPC components in a newly established esophageal cancer cell line from Persia (Iran), the high-risk region for esophageal cancer. Our results indicate overexpression of Hsc70 and downregulation of subunit alpha type-3 of proteasome, calpain small subunit 1, and eIF5A-1. Among these proteins, Hsc70 and eIF5A-1 are in direct interaction with NPC and involved in the nucleocytoplasmic exchange. Hsc70 plays a critical role as a chaperone in the formation of a cargo–receptor complex in nucleocytoplasmic transport. On the other hand, it is an NPC-associated protein that binds to nucleoporins and contributes in recycling of the nucleocytoplasmic transport receptors in mammals and affects transport of proteins between nucleus and cytoplasm. The other nuclear pore interacting protein: eIF5A-1 binds to the several nucleoporins and participates in nucleocytoplasmic transport. Altered expression of Hsc70 and eIF5A-1 may cause defects in nucleocytoplasmic transport and play a role in esophageal carcinogenesis.

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Authors and Affiliations

  1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Mehdi Moghanibashi & Kazem Parivar

  2. Department of Biochemistry, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

    Ferdous Rastgar Jazii, Zahra-Soheila Soheili & Aliasghar Karkhane

  3. Department of Pharmacology, College of Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada

    Ferdous Rastgar Jazii

  4. Department of Biology, Payam-Noor University, Tehran, Iran

    Maryam Zare

  5. Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

    Parisa Mohamadynejad

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  1. Mehdi Moghanibashi
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  2. Ferdous Rastgar Jazii
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  3. Zahra-Soheila Soheili
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  4. Maryam Zare
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  5. Aliasghar Karkhane
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  7. Parisa Mohamadynejad
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Correspondence to Mehdi Moghanibashi.

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Moghanibashi, M., Rastgar Jazii, F., Soheili, ZS. et al. Esophageal cancer alters the expression of nuclear pore complex binding protein Hsc70 and eIF5A-1. Funct Integr Genomics 13, 253–260 (2013). https://doi.org/10.1007/s10142-013-0320-9

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  • DOI: https://doi.org/10.1007/s10142-013-0320-9

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