Quantitative trait loci conferring resistance to Fusarium head blight in barley respond differentially to Fusarium graminearum infection
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Fusarium head blight (FHB), primarily caused by Fusarium graminearum, reduces grain yield and quality in barley. Resistance to FHB is partial and quantitatively inherited. Previously, major FHB resistant QTL were detected on barley chromosome 2H Bin 8 and 2H Bin 10, and another QTL for reduced deoxynivalenol (DON) accumulation was identified on chromosome 3H Bin 6. To develop an understanding of the molecular responses controlled by these loci, we examined DON and fungal biomass levels and the transcriptome differences in near-isogenic line (NIL) pairs carrying contrasting resistant and susceptible alleles at these QTL during F. graminearum infection. No overlap was found among the differentially accumulated transcripts of the three NIL pairs, indicating that the response to infection controlled by the resistance alleles at each QTL may be distinct. Transcripts showing differential accumulation between resistant and susceptible NILs were compared to results from previous wheat/barley–F. graminearum studies and integrated into a wheat/barley–F. graminearum interaction model.
KeywordsBarley Hordeum vulgare Fusarium head blight Near-isogenic line RNA profiling
We would like to thank Dr. R. Dill-Macky for providing the F. graminearum inoculum and Dr. Y. Dong for conducting the mycotoxin and ergosterol assays. We thank Dr. Z. Tu at the Supercomputing Institute for bioinformatics assistance. This work was supported by grants from the U.S. Wheat and Barley Scab Initiative (USDA-ARS) and Minnesota Small Grains Initiative to G.J.M. and a College of Food, Agriculture and Natural Resources Sciences Graduate Student Fellowship and University of Minnesota Graduate School Doctoral Dissertation Fellowship to H.J.
- Beaubien KA, Dill-Macky R, Dong Y, Steffenson BJ, Smith KP (2008) Characterizing barley near-isogenic lines for a DON QTL on chromosome 3H. In: Canty SM, Walton E, Clark A, Ellis D, Mundell J, Sanford DAV (eds) Proc national fusarium head blight forum. University of Kentucky, Indianapolis, pp 134–136Google Scholar
- Benjamini Y, Hochberg Y (1995) Controlling the false discovery rate: a practical and powerful approach to multiple testing. J Roy Stat Soc Ser B (Stat Method) 57:289–300Google Scholar
- Close TJ, Bhat PR, Lonardi S, Wu Y, Rostoks N, Ramsay L, Druka A, Stein N, Svensson JT, Wanamaker S, Bozdag S, Roose ML, Moscou MJ, Chao S, Varshney R, Szűcs P, Sato K, Hayes PM, Matthews DE, Kleinhofs A, Muehlbauer GJ, DeYoung J, Marshall DF, Madishetty K, Fenton RD, Condamine P, Graner A, Waugh R (2009) Development and implementation of high-throughput SNP genotyping in barley. BMC Genomics 10:582PubMedCrossRefGoogle Scholar
- Csiszár J, Szabó M, Illés E, Kurucz K (2002) Investigations of glutathione S-transferase and peroxidase activities in auxin heterotrophic and autotrophic tobacco calli under salt stress conditions. Proc 7th Hungarian Cong Plant Phys 46:79–80Google Scholar
- Desjardins AE (2006) Fusarium mycotoxins: chemistry, genetics, and biology. American Phytophthological Society, St. PaulGoogle Scholar
- Kandel S, Morant M, Benveniste I, Blee E, Werck-Reichhart D, Pinot F (2005) Cloning, functional expression, and characterization of CYP709C1, the first sub-terminal hydroxylase of long chain fatty acid in plants. Induction by chemicals and methyl jasmonate. J Biol Chem 280:35881–35889PubMedCrossRefGoogle Scholar
- Lemmens M, Scholz U, Berthiller F, Dall’Asta C, Koutnik A, Schumacher R, Adam G, Buerstmayr H, Mesterhazy A, Krska R (2005) The ability to detoxify the mycotoxin deoxynivalenol colocalizes with a major quantitative trait locus for Fusarium head blight resistance in wheat. Mol Plant-Microb Interact 18:1318–1324CrossRefGoogle Scholar
- Walter S, Brennan JM, Arunachalam C, Ansari KI, Hu X, Khan MR, Trognitz F, Trognitz B, Leonard G, Egan D, Doohan FM (2008) Components of the gene network associated with genotype-dependent response of wheat to the Fusarium mycotoxin deoxynivalenol. Funct Integr Genomics 8:421–427PubMedCrossRefGoogle Scholar