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HvNax3—a locus controlling shoot sodium exclusion derived from wild barley (Hordeum vulgare ssp. spontaneum)

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Abstract

Previous work identified the wild barley (Hordeum vulgare ssp. spontaneum) accession CPI-71284-48 as being capable of limiting sodium (Na+) accumulation in the shoots under saline hydroponic growth conditions. Quantitative trait locus (QTL) analysis using a cross between CPI-71284-48 and a selection of the cultivated barley (H. vulgare ssp. vulgare) cultivar Barque (Barque-73, a moderate Na+ excluder) attributed the control of the Na+ exclusion trait from CPI-71284-48 to a single locus on the short arm of chromosome 7H, which was named HvNax3. The locus reduced shoot Na+ accumulation by 10–25% in plants grown in 150 mM NaCl. Markers generated using colinearity with rice and Brachypodium, together with the analysis of introgression lines and F2 and F3 families, enabled HvNax3 to be mapped to a 1.3-cM interval. Genes from the corresponding rice and Brachypodium intervals encode 16 different classes of proteins and include several plausible candidates for HvNax3. The potential of HvNax3 to provide a useful trait contributing to salinity tolerance in cultivated barley is discussed.

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Acknowledgments

We thank Jason Eglinton, Stewart Coventry, and Julie Hayes for helpful discussions, Nilmini Dharmathilake and Anita Lapina for technical assistance, Jason Eglinton and Stewart Coventry for sharing unpublished data, the MPB-CRC for genetic resources, and codirectors of the Brachypodium Genome Sequencing Project for permission to use unpublished genomic sequence. This project was supported by a GRDC grant UA00090 to MT, a DBT grant (India) to NKG, research grant of the Institute of International Education (USA) to MNB, and by ARC, GRDC, and South Australian Government funding to ACPFG.

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Shavrukov, Y., Gupta, N.K., Miyazaki, J. et al. HvNax3—a locus controlling shoot sodium exclusion derived from wild barley (Hordeum vulgare ssp. spontaneum). Funct Integr Genomics 10, 277–291 (2010). https://doi.org/10.1007/s10142-009-0153-8

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