Fusarium graminearum-induced changes in gene expression between Fusarium head blight-resistant and susceptible wheat cultivars

Abstract

Fusarium head blight (FHB), primarily caused by Fusarium graminearum Schw., is a destructive disease of wheat (Triticum aestivum L.). Although several genes related to FHB resistance have been reported, global analysis of gene expression in response to FHB infection remains to be explored. The expression patterns of transcriptomes from wheat spikes of FHB-resistant cultivar Ning 7840 and susceptible cultivar Clark were monitored during a period of 72 h after inoculation (hai) with F. graminearum. Microarray analysis, coupled with suppression subtractive hybridization technique, identified 44 significantly differentially expressed genes between cv. Ning 7840 and cv. Clark. More differentially expressed genes were identified from susceptible libraries than from resistance libraries. The up-regulation of defense-related genes in Ning 7840 relative to cultivar Clark occurred during early fungal stress (3–12 hai). Three genes, with unknown function that were up-regulated in cv. Ning 7840 at most time points investigated, might play an important role in enhancing FHB resistance.

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Acknowledgements

This work was partially supported by the Great Plains Biotechnology Consortium and the US Wheat and Barley Scab Initiative. We thank Dr. Anne Desjardin for providing the GBZ 3639 strain of F. graminearum; the Oklahoma State University Microarray Core Facility for providing access to microarray equipment, which was supported by the grants from NSF (EOS-0132534) and NIH (2P20RR016478-04, 1P20RR16478-02, and 5P20RR15564-03); and Dr. Jianfa Bai at the Gene Expression Facility at Kansas State University for technical support on RT-PCR. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the US Department of Agriculture. This is contribution No. 05-277-5 from the Kansas Agricultural Experiment Station, Manhattan, KS.

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Correspondence to Guihua Bai.

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Table S1

Sequence of primers used for real time PCR (PDF 23 kb)

Figure S1

Fluorescence signal of eight cDNA templates amplified with wheat beta-actin primers (non-regulated control) indicate equal template concentrations (PDF 45kb)

Figure S2

Microarray (A) and real-time PCR (R) data in log2 ratios from a selected set of genes representing 6 clusters. Log2 ratios are on the Y-axis while the X-axis denotes the time point of inoculation. Letters to the right of the each graph indicate the gene cluster. A positive value indicates up-regulation of gene expression and a negative value indicates down-regulation of gene expression in resistant cv. Ning7840 relative to susceptible cv. Clark. RT-PCR values are from an average of two replicates (PDF 45 kb)

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Bernardo, A., Bai, G., Guo, P. et al. Fusarium graminearum-induced changes in gene expression between Fusarium head blight-resistant and susceptible wheat cultivars. Funct Integr Genomics 7, 69–77 (2007). https://doi.org/10.1007/s10142-006-0028-1

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Keywords

  • Fusarium head blight
  • Microarray
  • Gene expression
  • Wheat scab