Functional & Integrative Genomics

, Volume 2, Issue 4–5, pp 171–180 | Cite as

Global analysis of gene expression in yeast

  • Christine E. Horak
  • Michael Snyder
Review

Abstract.

In the past decade, there has been an intense effort to comprehensively catalogue the expressed genes in the yeast Saccharomyces cerevisiae and to determine the absolute and relative abundance of transcript and protein levels under different cellular conditions. Several methods have been developed to monitor gene expression: DNA microarray analysis, Serial Analysis of Gene Expression (SAGE), kinetic RT-PCR and monitoring expression of β-galactosidase fusion proteins. These techniques have been used to measure transcript and protein abundance in different developmental states and under different environmental stimuli. A wealth of expression data for yeast is now publicly available through several web sites. The expression information that exists has the obvious benefits of providing a better understanding of the gene expression patterns that accompany changes in a yeast cell's environmental and developmental states. This data has also, however, provided clues to unraveling the complicated questions surrounding gene regulation: why and how is gene expression controlled?

Microarray SAGE Kinetic RT-PCR Beta-galactosidase fusion proteins 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Christine E. Horak
    • 1
  • Michael Snyder
    • 1
  1. 1.Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USAUSA

Personalised recommendations