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Identification of Microsatellite Repeats in Turbot (Scophthalmus maximus) and Dover Sole (Solea solea) using a RAPD-Based Technique: Characterization of Microsatellite Markers in Dover Sole

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Abstract:

We have used a RAPD-based technique to identify several microsatellite repeats in turbot (Scophthalmus maximus) and Dover sole (Solea solea) and report the characterization of six novel polymorphic microsatellite markers for Dover sole. These are the first such markers to be developed for this flatfish species. They exhibit much higher levels of heterozygosity than those previously observed with allozyme loci and should prove useful in addressing population genetic questions as well as more fundamental aquaculture-related questions.

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Authors and Affiliations

  1. School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, U.K., , , , , , GB

    Arati Iyengar, Sanit Piyapattanakorn & Norman Maclean

  2. CEFAS Weymouth Laboratory, Weymouth, Dorset DT4 8UB, U.K., , , , , , GB

    David M. Stone, Diana A. Heipel & Bari R. Howell

  3. CEFAS Conwy Laboratory, Conwy, North Wales LL32 8UB, U.K., , , , , , GB

    Stephen M. Baynes

Authors
  1. Arati Iyengar
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  2. Sanit Piyapattanakorn
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  3. David M. Stone
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  4. Diana A. Heipel
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  5. Bari R. Howell
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  6. Stephen M. Baynes
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  7. Norman Maclean
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Received ; accepted July 13, 1999.

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Iyengar, A., Piyapattanakorn, S., Stone, D. et al. Identification of Microsatellite Repeats in Turbot (Scophthalmus maximus) and Dover Sole (Solea solea) using a RAPD-Based Technique: Characterization of Microsatellite Markers in Dover Sole. Mar. Biotechnol. 2, 49–56 (2000). https://doi.org/10.1007/s101269900007

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  • DOI: https://doi.org/10.1007/s101269900007

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