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A Simple, Reliable, and Fast Protocol for Thraustochytrid DNA Extraction

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Abstract:

DNA extraction of thraustochytrids, common marine unicellular organisms, is usually accomplished by either the cetyltrimethylammonium bromide (CTAB) or proteinase K protocols. A novel lysis buffer protocol for thraustochytrid total DNA extraction is described. The average isolated total DNA is 20 to 40 kb, and DNA samples are suitable for a variety of uses including 18S–ribosomal DNA polymerase chain reaction, restriction enzyme digestions, and amplified fragment length polymorphism analyses. The new protocol is also faster than the other protocols.

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Authors and Affiliations

  1. Minerva Center for Marine Invertebrate Immunology and Developmental Biology, Israel Oceanographic and Limnological Research, National Institute of Oceanography, P.O. Box 8030, Tel Shikmona, Haifa 31080, Israel, , , , , , IL

    Caiqing Mo & Baruch Rinkevich

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  1. Caiqing Mo
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  2. Baruch Rinkevich
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Received July 31, 2000; accepted November 2, 2000.

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Mo, C., Rinkevich, B. A Simple, Reliable, and Fast Protocol for Thraustochytrid DNA Extraction. Mar. Biotechnol. 3, 100–102 (2001). https://doi.org/10.1007/s101260000069

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  • DOI: https://doi.org/10.1007/s101260000069

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