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Temporal Gene Expression Signature of Plasma Extracellular Vesicles-MicroRNAs from Post-Smolt Coho Salmon Challenged with Piscirickettsia salmonis

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Abstract

Piscirickettsiosis is the most important bacterial disease in the Chilean salmon industry, which has borne major economic losses due to failure to control it. Cells use extracellular vesicles (EVs) as an inter-cellular communicators to deliver several factors (e.g., microRNAs) that may regulate the responses of other cells. However, there is limited knowledge about the identification and characterization of EV-miRNAs in salmonids or the effect of infections on these. In this study, Illumina sequencing technology was used to identify Coho salmon plasma EV-miRNAs upon Piscirickettsia salmonis infection at four different time points. A total of 118 novels and 188 known EV-miRNAs, including key immune teleost miRNAs families (e.g., miR-146, miR-122), were identified. A total of 245 EV-miRNAs were detected as differentially expressed (FDR < 5%) in terms of control, with a clear down-regulation pattern throughout the disease. KEGG enrichment results of EV-miRNAs target genes showed that they were grouped mainly in cellular, stress, inflammation and immune responses. Therefore, it is hypothesized that P. salmonis could potentially benefit from unbalanced modulation response of Coho salmon EV-miRNAs in order to promote a hyper-inflammatory and compromised immune response through the suppression of different key immune host miRNAs during the course of the infection, as indicated by the results of this study.

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Sequence data file has been deposited in the Sequence Read Archive (SRA), accession number National Center for Biotechnology Information (NCBI) SRA# PRJNA627938.

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Funding

This research was supported by CORFO-INNOVA Chile 12IDL2-16192 and DICYT 022043VS, Vicerrectoría de Investigación, Desarrollo e Innovacion. K.K.G thank the Fellowship USA 1899 from the Universidad de Santiago de Chile.

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Authors and Affiliations

  1. Laboratory of Molecular Ecology, Genomics and Evolutionary Studies, Department of Biology, Universidad de Santiago de Chile, Santiago, Chile

    Francisco Leiva, Scarlet Bravo, Killen Ko Garcia & Rodrigo Vidal

  2. Benchmark Animal Health Chile, Santa Rosa 560 Of.26, Puerto Varas, Chile

    Javier Moya

  3. IDEVAC SpA, Francisco Bilbao 1129 Of. 306, Osorno, Chile

    Osiel Guzman

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  1. Francisco Leiva
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  2. Scarlet Bravo
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  3. Killen Ko Garcia
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  4. Javier Moya
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  5. Osiel Guzman
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  6. Rodrigo Vidal
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Contributions

F.L. and R.V designed research; S.B., K. K. G., and F.L., developed experimental protocols and bioinformatics pipelines; J.M. conducted the sampling; and R.V., F.L., and K. K. G, wrote the paper. All authors reviewed the manuscript.

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Correspondence to Rodrigo Vidal.

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The totality of the animal experiments in this study performed in agreement with the Institutional Ethics Committee of the Universidad de Santiago (USCH-1) and AVMA- 2019 guidelines.

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The authors declare no competing interests. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

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Leiva, F., Bravo, S., Garcia, K.K. et al. Temporal Gene Expression Signature of Plasma Extracellular Vesicles-MicroRNAs from Post-Smolt Coho Salmon Challenged with Piscirickettsia salmonis. Mar Biotechnol 23, 602–614 (2021). https://doi.org/10.1007/s10126-021-10049-0

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