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Development of a Method for Fucoxanthin Production Using the Haptophyte Marine Microalga Pavlova sp. OPMS 30543

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Abstract

The natural pigment fucoxanthin has attracted global attention because of its superior antioxidant properties. The haptophyte marine microalgae Pavlova spp. are assumed to be promising industrial fucoxanthin producers as their lack of a cell wall could facilitate the commercialization of cultured cells as a whole food. This study screened promising Pavlova strains with high fucoxanthin content to develop an outdoor cultivation method for fucoxanthin production. Initial laboratory investigations of P. pinguis NBRC 102807, P. lutheri NBRC 102808, and Pavlova sp. OPMS 30543 identified OPMS 30543 as having the highest fucoxanthin content. The culture conditions were optimized for OPMS 30543. Compared with f/2 and Walne’s media, the use of Daigo’s IMK medium led to the highest biomass production and highest fucoxanthin accumulation. The presence of seawater elements in Daigo’s IMK medium was necessary for the growth of OPMS 30543. OPMS 30543 was then cultured outdoors using acrylic pipe photobioreactors, a plastic bag, an open tank, and a raceway pond. Acrylic pipe photobioreactors with small diameters enabled the highest biomass production. Using an acrylic pipe photobioreactor with 60-mm diameter, a fucoxanthin productivity of 4.88 mg/L/day was achieved in outdoor cultivation. Thus, this study demonstrated the usefulness of Pavlova sp. OPMS 30543 for fucoxanthin production in outdoor cultivation.

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Data Availability

The data supporting the findings of this study are available within this article or from the corresponding author upon reasonable request. Pavlova pinguis NBRC 102807 and Pavlova lutheri NBRC 102808 can be obtained from the National Biological Resource Center (NBRC).

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Acknowledgements

The authors thank Dr. Takeshi Fujiwara, Dr. Takafumi Watanabe, and Ms. Yuko Koizumi for their technical assistance. We would like to thank NBRC for supplying Pavlova pinguis NBRC 102807 and Pavlova lutheri NBRC 102808.

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Authors and Affiliations

  1. Graduate School of Innovation, Science and Technology, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan

    Akihiko Kanamoto, Erina Yoshida, Tomohisa Hasunuma & Akihiko Kondo

  2. OP Bio Factory Co., Ltd., 5-8 Aza-Suzaki, Uruma, Okinawa, 904-2234, Japan

    Akihiko Kanamoto

  3. Engineering Biology Research Center, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan

    Yuichi Kato, Tomohisa Hasunuma & Akihiko Kondo

  4. RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro, Tsurumi, Yokohama, Kanagawa, 230-0045, Japan

    Akihiko Kondo

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  1. Akihiko Kanamoto
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  2. Yuichi Kato
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  3. Erina Yoshida
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  4. Tomohisa Hasunuma
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  5. Akihiko Kondo
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Contributions

A. Kanamoto designed the study, conducted the experiments, and drafted the manuscript. Y. K., E. Y., T. H., and A. Kondo commented on the study, helped interpret results, and revised the manuscript. All authors approved the final version of the manuscript.

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Correspondence to Tomohisa Hasunuma.

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The authors declare that they have no competing interests.

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A. Kanamoto was a CEO of OP Bio Factory at the time this study was conducted. A. Kanamoto participated in the experiments as a representative of OP Bio Factory. The corresponding author has full access to all the data in the study and is completely responsible for the data and its accuracy.

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Kanamoto, A., Kato, Y., Yoshida, E. et al. Development of a Method for Fucoxanthin Production Using the Haptophyte Marine Microalga Pavlova sp. OPMS 30543. Mar Biotechnol 23, 331–341 (2021). https://doi.org/10.1007/s10126-021-10028-5

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