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Characterization of Macroscopic Colony-Forming Filamentous Cyanobacteria from Okinawan Coasts as Potential Sources of Bioactive Compounds

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Abstract

Marine macroscopic colony-forming filamentous (MMCFF) cyanobacteria are considered as prolific producers of bioactive compounds. Thus, knowledge of the diversity of MMCFF cyanobacteria as related to bioactive compound production has become very important. However, basic taxonomic studies of MMCFF cyanobacteria are lacking. Many cyanobacterial taxa are still misidentified or undescribed. In this study, a total of 32 cyanobacterial colonies from nine coastal regions of Okinawa Prefecture were investigated for a diversity assessment. A polyphasic approach including morphological and molecular studies based on 16S rRNA gene sequences was performed to characterize Okinawan MMCFF cyanobacteria. Both morphological and molecular phylogenetic results showed that MMCFF cyanobacteria from Okinawan coasts are very diverse. We found morphotypes of Lyngbya-like, Phormidium-like, and Leptolyngbya-like groups among Okinawan cyanobacterial samples. Genetically, samples were distributed in various clades in the phylogenetic tree, including within Moorena, Okeania, Caldora, Neolyngbya, Dapis, as well as several unknown clades. In addition, cytotoxic activities of three samples from Kiyan coast were tested against HeLa cells. All three crude extracts of these samples showed strong cytotoxic activity with IC50 < 1 μg/ml.

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Acknowledgments

The authors would like to thank Associate Professor James Davis Reimer for manuscript proofreading and comments. Part of this study was supported by the Okinawa Science and Technology Innovation System Construction Program.

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Authors and Affiliations

  1. Graduate School of Engineering and Science, University of the Ryukyus, Nishihara, Okinawa, Japan

    Handung Nuryadi

  2. Department of Material and Life Chemistry, Kanagawa University, Yokohama, Kanagawa, Japan

    Shimpei Sumimoto

  3. Faculty of Education, University of the Ryukyus, Nishihara, Okinawa, Japan

    Toshiaki Teruya

  4. Department of Chemistry, Keio University, Yokohama, Kanagawa, Japan

    Kiyotake Suenaga

  5. Faculty of Science, University of the Ryukyus, Nishihara, Okinawa, Japan

    Shoichiro Suda

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  1. Handung Nuryadi
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  2. Shimpei Sumimoto
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Nuryadi, H., Sumimoto, S., Teruya, T. et al. Characterization of Macroscopic Colony-Forming Filamentous Cyanobacteria from Okinawan Coasts as Potential Sources of Bioactive Compounds. Mar Biotechnol 22, 824–835 (2020). https://doi.org/10.1007/s10126-020-10010-7

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