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In Vitro Antitumor Potential of Sulfated Polysaccharides from Seaweed Caulerpa cupressoides var. flabellata

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Abstract

Seaweeds are important source of bioactive compounds, including sulfated polysaccharides (SP). Because of their structural heterogeneity, these compounds are promising sources of anticancer compounds. SP from brown and red seaweeds have shown antimelanoma activity in different in vitro and in vivo models. However, SP from green seaweed are still poorly evaluated. Therefore, SP were extracted from the green alga Caulerpa cupressoides var. flabellata, and their antiproliferative, anti-migratory, and inhibitory effect on melanin production on B16-F10 melanoma cells was evaluated. Cell assays, including flow cytometry, demonstrated that SP (100–1000 μg mL−1) are non-cytotoxic, do not induce apoptosis or necrosis, and do not interfere with cell cycle. However, SP (1000 μg mL−1) were found to significantly inhibit cell colony formation (80–90%), cell migration (40–75%), and melanin production (~ 20%). In summary, these results showed that SP inhibited important melanoma development events without cytotoxicity effects, suggesting that C. cupressoides may be an important source of SP with antitumor properties.

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Acknowledgments

The authors wish to thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for the financial support. Hugo Alexandre O. Rocha is CNPq fellowship-honored researcher. Laís Cristina G. F. Palhares and Cynthia Haynara F. Silva received a scholarship from the CAPES. Diego de A. Sabry received a scholarship from the PNPD-CAPES. The authors would like to thank the Department of Biochemistry, Institute of Tropical Medicine of Rio Grande do Norte, and Brain Institute at Universidade Federal do Rio Grande do Norte for letting us use the cell culture room, flow cytometer, and videomicroscope, respectively. This research was presented at Programa de Pós-Graduação em Ciências da Saúde at the Universidade Federal do Rio Grande do Norte, as part of the doctoral thesis of Jefferson da S. Barbosa.

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Authors and Affiliations

  1. Laboratório de Biotecnologia de Polímeros Naturais (BIOPOL), Departamento de Bioquímica, Centro de Biociências, Universidade Federal do Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte, 59078-970, Brazil

    Jefferson da Silva Barbosa, Cynthia Haynara Ferreira Silva, Diego Araujo Sabry & Hugo Alexandre Oliveira Rocha

  2. Programa de Pós-graduação em Ciências da Saúde, Universidade Federal do Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte, 59012-570, Brazil

    Jefferson da Silva Barbosa & Hugo Alexandre Oliveira Rocha

  3. Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Norte (IFRN), Campus São Gonçalo do Amarante, São Gonçalo do Amarante, Rio Grande do Norte, 59291-727, Brazil

    Jefferson da Silva Barbosa

  4. Programa de Pós-graduação em Bioquímica, Departamento de Bioquímica, Centro de Biociências, Universidade Federal do Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte, 59012-570, Brazil

    Laís Cristina Gusmão Ferreira Palhares, Cynthia Haynara Ferreira Silva, Suely Ferreira Chavante & Hugo Alexandre Oliveira Rocha

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  1. Jefferson da Silva Barbosa
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  2. Laís Cristina Gusmão Ferreira Palhares
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  3. Cynthia Haynara Ferreira Silva
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  6. Hugo Alexandre Oliveira Rocha
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da Silva Barbosa, J., Palhares, L.C.G.F., Silva, C.H.F. et al. In Vitro Antitumor Potential of Sulfated Polysaccharides from Seaweed Caulerpa cupressoides var. flabellata. Mar Biotechnol 23, 77–89 (2021). https://doi.org/10.1007/s10126-020-10004-5

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  • Issue Date:

  • DOI: https://doi.org/10.1007/s10126-020-10004-5

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