Skip to main content
SpringerLink
Log in

Identification of Functional SSR Markers in Freshwater Ornamental Shrimps Neocaridina denticulata Using Transcriptome Sequencing

  • Original Article
  • Published:
Marine Biotechnology Aims and scope Submit manuscript

Abstract

The amazing colors and patterns are fascinating characteristics in all of the aquarium species. However, genetic and breeding molecular investigations of ornamental shrimps are rather limited. Here, we present the first transcriptomic analysis and application of microsatellites based on the chromatophore-encoded genes of Neocaridina denticulata to assist freshwater ornamental shrimp germplasm enhancement and its extensive applications. A total of 65,402 unigenes were annotated, and 4706 differentially expressed genes were screened and identified between super red shrimp and chocolate shrimp strains. Several gene ratios were examined to put in perspective possible genetic markers for the different strains of normal pigmentation development, including flotillin-2-like, keratin, the G protein–coupled receptor Mth2-like, annexin A7, and unconventional myosin-IXb-like. Five simple sequence repeat markers were effective for colored shrimps and were used to develop a marker-assisted selection platform for systematic breeding management program to maintain genetic diversity of the species. These markers could also be used to assist the identification of pure strains and increase the genetic stability of ornamental shrimp color phenotypes. Consequently, our results of microsatellite marker development are valuable for assisting shrimp genetic and selection breeding studies on freshwater ornamental shrimp and related crystal shrimp species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Andriantahina F, Liu X, Huang H (2013) Genetic map construction and quantitative trait locus (QTL) detection of growth-related traits in Litopenaeus vannamei for selective breeding applications. PLoS One 8:e75206

    CAS  PubMed  PubMed Central  Google Scholar 

  • Ariyanathan G, Serebiah JS (2016) Selective and interbreeding of freshwater ornamental shrimps with endemic aquarium plants. Int J Curr Res Biosci Plant Biol 3:144–150

    Google Scholar 

  • Ben J, Lim TM, Phang VP, Chan WK (2003) Cloning and tissue expression of 6-pyruvoyl tetrahydropterin synthase and xanthine dehydrogenase from Poecilia reticulata. Mar Biotechnol 5:568–578

    CAS  Google Scholar 

  • Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:314–331

    CAS  PubMed  PubMed Central  Google Scholar 

  • Brownawell AM, Creutz CE (1997) Calcium-dependent binding of sorcin to the N-terminal domain of synexin (annexin VII). J Biol Chem 272:22182–22190

    CAS  PubMed  Google Scholar 

  • Castle J, Garrett-Engele P, Armour CD, Duenwald SJ, Loerch PM, Meyer MR, Schadt EE, Stoughton R, Parrish ML, Shoemaker DD, Johnson JM (2003) Optimization of oligonucleotide arrays and RNA amplification protocols for analysis of transcript structure and alternative splicing. Genome Biol 4:1

    Google Scholar 

  • Chen X, Mei J, Wu J, Jing J, Ma W, Zhang J, Dan C, Wang W, Gui JF (2015) A comprehensive transcriptome provides candidate genes for sex determination/differentiation and SSR/SNP markers in yellow catfish. Mar Biotechnol 17:190–198

    CAS  Google Scholar 

  • Chen J, Glémin S, Lascoux M (2017) Genetic diversity and the efficacy of purifying selection across plant and animal species. Mol Biol Evol 34:1417–1428

    CAS  PubMed  Google Scholar 

  • Cheung MK, Yip HY, Nong W, Law PT, Chu KH, Kwan HS, Hui JH (2015) Rapid change of microbiota diversity in the gut but not the hepatopancreas during gonadal development of the new shrimp model Neocaridina denticulata. Mar Biotechnol 17:811–819

    CAS  Google Scholar 

  • Chieregatti E, Gärtner A, Stöffler HE, Bähler M (1998) Myr 7 is a novel myosin IX-RhoGAP expressed in rat brain. J Cell Sci 111:3597–3608

    CAS  PubMed  Google Scholar 

  • Chow LH, Ma KY, Hui JHL, Chu KH (2018) Isolation and characterization of polymorphic microsatellite loci for Caridina cantonensis and transferability across eight confamilial species (Atyidae, Decapoda). Zool Stud 57:e19

    PubMed  PubMed Central  Google Scholar 

  • Conesa A, Gotz S, Garcia-Gomez JM, Terol J, Talon M, Robles M (2005) Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics 21:3674–3676

    CAS  PubMed  Google Scholar 

  • Cornejo-Granados F, Gallardo-Becerra L, Leonardo-Reza M, Ochoa-Romo JP, Ochoa-Leyva A (2018) A meta-analysis reveals the environmental and host factors shaping the structure and function of the shrimp microbiota. PeerJ 6:e5382

    PubMed  PubMed Central  Google Scholar 

  • Curran K, Lister JA, Kunkel GR, Prendergast A, Parichy DM, Raible DW (2010) Interplay between Foxd3 and Mitf regulates cell fate plasticity in the zebrafish neural crest. Dev Biol 344:107–118

    CAS  PubMed  PubMed Central  Google Scholar 

  • Ertl NG, Elizur A, Brooks P, Kuballa AV, Anderson TA, Knibb WR (2013) Molecular characterisation of colour formation in the prawn Fenneropenaeus merguiensis. PLoS One 8:e56920

    CAS  PubMed  PubMed Central  Google Scholar 

  • Fu L, Niu B, Zhu Z, Wu S, Li W (2012) CD-HIT: accelerated for clustering the next-generation sequencing data. Bioinformatics 28:3150-3152

  • García-Borrón JC, Sánchez-Laorden BL, Jiménez-Cervantes C (2005) Melanocortin-1 receptor structure and functional regulation. Pigment Cell Res 18:393–410

  • Ghaffari N, Sanchez-Flores A, Doan R, Garcia-Orozco KD, Chen PL, Ochoa-Leyva A, Lopez-Zavala AA, Carrasco JS, Hong C, Brieba LG, Rudiño-Piñera E, Blood PD, Sawyer JE, Johnson CD, Dindot SV, Sotelo-Mundo RR, Criscitiello MF (2014) Novel transcriptome assembly and improved annotation of the whiteleg shrimp (Litopenaeus vannamei), a dominant crustacean in global seafood mariculture. Sci Rep 4:7081

    CAS  PubMed  PubMed Central  Google Scholar 

  • Ha T, Naysmith L, Waterston K, Oh C, Weller R, Rees JL (2003) Defining the quantitative contribution of the melanocortin 1 receptor (MC1R) to variation in pigmentary phenotype. Ann N Y Acad Sci 994:339–347

    CAS  PubMed  Google Scholar 

  • Hu XL, Niu JJ, Meng Q, Chai YH, Chu KH, Chan KM (2019) Effects of two juvenile hormone analogue insecticides, fenoxycarb and methoprene, on Neocaridina davidi. Environ Pollut 253:89–99

    CAS  PubMed  Google Scholar 

  • Hung MS, Chan TY, Yu HP (1993) Atyid shrimps (Decapoda: Caridea) of Taiwan, with descriptions of three new species. J Crustac Biol 13:481–503

    Google Scholar 

  • Jiang J, Yuan X, Qiu Q, Huang G, Jiang Q, Fu P, Zhang Y, Jia Y, Yang X, Jiang H (2019) Comparative transcriptome analysis of gonads for the identification of sex-related genes in giant freshwater prawns (Macrobrachium rosenbergii) using RNA sequencing. Genes 10:E1035

    PubMed  Google Scholar 

  • Kanehisa M, Araki M, Goto S, Hattori M, Hirakawa M, Itoh M, Katayama T, Kawashima S, Okuda S, Tokimatsu T, Yamanishi Y (2008) KEGG for linking genomes to life and the environment. Nucleic Acids Res 36:D480–D484

    CAS  PubMed  Google Scholar 

  • Kenny NJ, Sin YW, Shen X, Zhe Q, Wang W, Chan TF, Tobe SS, Shimeld SM, Chu KH, Hui JH (2014) Genomic sequence and experimental tractability of a new decapod shrimp model, Neocaridina denticulata. Mar Drugs 12:1419–1437

    CAS  PubMed  PubMed Central  Google Scholar 

  • Kottler VA, Fadeev A, Weigel D, Dreyer C (2013) Pigment pattern formation in the guppy, Poecilia reticulata, involves the Kita and Csf1ra receptor tyrosine kinases. Genetics 194:631–646

    CAS  PubMed  PubMed Central  Google Scholar 

  • Lee JH (2017) Quantitative approaches for investigating the spatial context of gene expression. Wiley Interdiscip Rev Syst Biol Med 9:e1369

    Google Scholar 

  • Leu JH, Chen SH, Wang YB, Chen YC, Su SY, Lin CY, Ho JM, Lo CF (2011) A review of the major penaeid shrimp EST studies and the construction of a shrimp transcriptome database based on the ESTs from four penaeid shrimp. Mar Biotechnol 13:608–621

    CAS  Google Scholar 

  • Levitt-Barmats Y, Yanai Z, Cohen TM, Shenkar N (2019) Life-history traits and ecological characteristics of the ornamental shrimp Neocaridina denticulata (De Haan, 1844), recently introduced into the freshwater systems of Israel. Aquat Invasions 14:684–702

    Google Scholar 

  • Lynn Lamoreux M, Kelsh RN, Wakamatsu Y, Ozato K (2005) Pigment pattern formation in the medaka embryo. Pigment Cell Res 18:64–73

    CAS  PubMed  Google Scholar 

  • McMichael BK, Scherer KF, Franklin NC, Lee BS (2014) The RhoGAP activity of myosin IXB is critical for osteoclast podosome patterning, motility, and resorptive capacity. PLoS One 9:e87402

    PubMed  PubMed Central  Google Scholar 

  • McNamara JC, Milograna SR (2015) Adaptive color change and the molecular endocrinology of pigment translocation in crustacean chromatophores. In: The Natural History of the Crustacea. 4:68–102

  • Milograna SR, Ribeiro MR, Baqui MM, McNamara JC (2014) Pigment granule translocation in red ovarian chromatophores from the palaemonid shrimp Macrobrachium olfersi (Weigmann, 1836): functional roles for the cytoskeleton and its molecular motors. Comp Biochem Physiol A Mol Integr Physiol 178:90–101

    CAS  PubMed  Google Scholar 

  • Milograna SR, Ribeiro MR, Bell FT, McNamara JC (2016) Pigment translocation in caridean shrimp chromatophores: receptor type, signal transduction, second messengers, and cross talk among multiple signaling cascades. J Exp Zool A Ecol Genet Physiol 325:565–580

    CAS  PubMed  Google Scholar 

  • Mortazavi A, Williams BA, McCue K, Schaeffer L, Wold B (2008) Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nat Methods 5:621–628

    CAS  PubMed  Google Scholar 

  • Mykles DL, Hui JH (2015) Neocaridina denticulata: a decapod crustacean model for functional genomics. Integr Comp Biol 55:891–897

    PubMed  Google Scholar 

  • Negisho T, Gemeda G, Du Laing G, Wakjira M, Janssens GPJ (2019) Diversity in micromineral distribution within the body of ornamental fish species. Biol Trace Elem Res. https://doi.org/10.1007/s12011-019-01983-1

  • Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89:583–590

    CAS  PubMed  PubMed Central  Google Scholar 

  • Neumann-Giesen C, Fernow I, Amaddii M, Tikkanen R (2007) Role of EGF-induced tyrosine phosphorylation of reggie-1/flotillin-2 in cell spreading and signaling to the actin cytoskeleton. J Cell Sci 120:395–406

    CAS  PubMed  Google Scholar 

  • Nguyen NH, Quinn J, Powell D, Elizur A, Thoa NP, Nocillado J, Lamont R, Remilton C, Knibb W (2014) Heritability for body colour and its genetic association with morphometric traits in Banana shrimp (Fenneropenaeus merguiensis). BMC Genet 15:132

    PubMed  PubMed Central  Google Scholar 

  • Nong W, Chai ZYH, Jiang X, Qin J, Ma KY, Chan KM, Chan TF, Chow BKC, Kwan HS, Wong CKC, Qiu JW, Hui JHL, Chu KH (2020) A crustacean annotated transcriptome (CAT) database. BMC Genomics 21:32

    PubMed  PubMed Central  Google Scholar 

  • Nur FAH, Christianus A (2013) Breeding and life cycle of Neocaridina denticulata sinensis (Kemp, 1918). Asian J Anim Vet Adv 8:108–115

    Google Scholar 

  • Pan G, Yang J (2010) Analysis of microsatellite DNA markers reveals no genetic differentiation between wild and hatchery populations of Pacific threadfin in Hawaii. Int J Biol Sci 6:827–833

    CAS  PubMed  PubMed Central  Google Scholar 

  • Parichy DM, Rawls JF, Pratt SJ, Whitfield TT, Johnson SL (1999) Zebrafish sparse corresponds to an orthologue of c-kit and is required for the morphogenesis of a subpopulation of melanocytes, but is not essential for hematopoiesis or primordial germ cell development. Development 126:3425–3436

    CAS  PubMed  Google Scholar 

  • Parichy DM, Mellgren EM, Rawls JF, Lopes SS, Kelsh RN, Johnson SL (2000) Mutational analysis of endothelin receptor b1 (rose) during neural crest and pigmentpattern development in the zebrafish Danio rerio. Dev Biol 227:294–306

    CAS  PubMed  Google Scholar 

  • Patoka J, Bláha M, Devetter M, Rylková K, Čadková Z, Kalous L (2015) Aquarium hitchhikers: attached commensals imported with freshwater shrimps via the pet trade. Biol Invasions 18:457–461

    Google Scholar 

  • Pinnegar JK, Murray JM (2019) Understanding the United Kingdom marine aquarium trade - a mystery shopper study of species on sale. J Fish Biol 94:917–924

    PubMed  Google Scholar 

  • Riento K, Frick M, Schafer I, Nichols BJ (2009) Endocytosis of flotillin-1 and flotillin-2 is regulated by Fyn kinase. J Cell Sci 122:912–918

    CAS  PubMed  PubMed Central  Google Scholar 

  • Sajeela KA, Gopalakrishnan A, Basheer VS, Mandal A, Bineesh KK, Grinson G, Gopakumar SD (2019) New insights from nuclear and mitochondrial markers on the genetic diversity and structure of the Indian white shrimp Fenneropenaeus indicus among the marginal seas in the Indian Ocean. Mol Phylogenet Evol 136:53–64

    CAS  PubMed  Google Scholar 

  • Shih HT, Cai Y (2007) Two new species of the land-locked freshwater shrimp genus, Neocaridina Kubo, 1938 (Decapoda: Caridea: Atyidae), from Taiwan, with notes on speciation on the island. Zool Stud 46:680–694

    CAS  Google Scholar 

  • Sin YW, Kenny NJ, Qu Z, Chan KW, Chan KW, Cheong SP, Leung RW, Chan TF, Bendena WG, Chu KH, Tobe SS, Hui JH (2015) Identification of putative ecdysteroid and juvenile hormone pathway genes in the shrimp Neocaridina denticulata. Gen Comp Endocrinol 214:167–176

    CAS  PubMed  Google Scholar 

  • Sung HH, Chiu YW, Wang SY, Chen CM, Huang DJ (2014) Acute toxicity of mixture of acetaminophen and ibuprofen to green neon shrimp, Neocaridina denticulate. Environ Toxicol Pharmacol 38:8–13

    CAS  PubMed  Google Scholar 

  • Suzuki T, Kanou Y (2014) Two distinct arginine kinases in Neocaridina denticulate: psychrophilic and mesophilic enzymes. Int J Biol Macromol 67:433–438

    CAS  PubMed  Google Scholar 

  • Tang D, Shi X, Guo H, Bai Y, Shen C, Zhang Y, Wang Z (2020) Comparative transcriptome analysis of the gills of Procambarus clarkii provides novel insights into the immune-related mechanism of copper stress tolerance. Fish Shellfish Immunol 96:32–40

    CAS  PubMed  Google Scholar 

  • Thiel T, Michalek W, Varshney RK, Graner A (2003) Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.). Theor Appl Genet 106:411–422

    CAS  PubMed  Google Scholar 

  • Tuma MC, Gelfand VI (1999) Molecular mechanisms of pigment transport in melanophores. Pigment Cell Res 12:283–294

    CAS  PubMed  Google Scholar 

  • van der Salm AL, Metz JR, Wendelaar Bonga SE, Flik G (2005) Alpha-MSH, the melanocortin-1 Receptor and Background Adaptation in the Mozambique Tilapia, Oreochromis Mossambicus. Gen Comp Endocrinol 144:140–149

  • von Rintelen K, Page TJ, Cai Y, Roe K, Stelbrink B, Kuhajda BR, Iliffe TM, Hughes J, von Rintelen T (2012) Drawn to the dark side: a molecular phylogeny of freshwater shrimps (Crustacea: Decapoda: Caridea: Atyidae) reveals frequent cave invasions and challenges current taxonomic hypotheses. Mol Phylogenet Evol 63:82–96

    Google Scholar 

  • Wade NM, Melville-Smith R, Degnan BM, Hall MR (2008) Control of shell colour changes in the lobster, Panulirus cygnus. J Exp Biol 211:1512–1519

    PubMed  Google Scholar 

  • Wade NM, Anderson M, Sellars MJ, Tume RK, Preston NP, Glencross BD (2012) Mechanisms of colour adaptation in the prawn Penaeus monodon. J Exp Biol 215:343–350

    PubMed  Google Scholar 

  • Wang J, Yu X, Zhao K, Zhang Y, Tong J, Peng Z (2012) Microsatellite development for an endangered bream Megalobrama pellegrini (Teleostei, Cyprinidae) using 454 sequencing. Int J Mol Sci 13:3009–3021

    CAS  PubMed  PubMed Central  Google Scholar 

  • Wang W, Yang S, Wang C, Shi L, Guo H, Chan S (2017) Gill transcriptomes reveal involvement of cytoskeleton remodeling and immune defense in ammonia stress response in the banana shrimp Fenneropenaeus merguiensis. Fish Shellfish Immunol 71:319–328

    CAS  PubMed  Google Scholar 

  • Wang J, Ge Q, Li J, Gao H, Li J, Zhao F (2018) Identification of novel EST-SSR markers by transcriptome sequencing in ridgetail white prawn Exopalaemon carinicauda. Genes Genom 40:207–215

    CAS  Google Scholar 

  • Wang F, Li S, Xiang J, Li F (2019) Transcriptome analysis reveals the activation of neuroendocrine-immune system in shrimp hemocytes at the early stage of WSSV infection. BMC Genomics 20:247

    PubMed  PubMed Central  Google Scholar 

  • Wirth JA, Jensen KA, Post PL, Bement WM, Mooseker MS (1996) Human myosin-IXb, an unconventional myosin with a chimerin-like rho/rac GTPase-activating protein domain in its tail. J Cell Sci 109:653–661

    CAS  PubMed  Google Scholar 

  • Woolley TE, Maini PK, Gaffney EA (2014) Is pigment cell pattern formation in zebrafish a game of cops and robbers? Pigment Cell Melanoma Res 27:686–687

    PubMed  Google Scholar 

  • Wu JP, Li MH (2015) Inhibitory effects of pain relief drugs on neurological enzymes: implications on their potential neurotoxicity to aquatic animals. Environ Toxicol Pharmacol 39:898–905

    CAS  PubMed  Google Scholar 

  • Xu G, Du F, Nie Z, Xu P, Gu R (2014) Complete mitochondrial genome of Caridina nilotica gracilipes. Mitochondrial DNA 4:1–2

    CAS  Google Scholar 

  • Yamada S, Tanaka Y, Sameshima M, Ito Y (1990) Pigmentation of prawn (Penaeus japonicus) with carotenoids: I. effect of dietary astaxanthin, β-carotene and canthaxanthin on pigmentation. Aquaculture 87:323–330

    CAS  Google Scholar 

  • Yeh FC, Yang R, Boyle TJ, Ye Z, Xiyan JM (2000) POPGENE32, version 1.32. Microsoft Window-based freeware for population genetic analysis. University of Alberta, Edmonton, Canada

    Google Scholar 

  • Yu YQ, Yang WJ, Yang JS (2014) The complete mitogenome of the Chinese swamp shrimp Neocaridina denticulata sinensis Kemp 1918 (Crustacea: Decapoda: Atyidae). Mitochondrial DNA 25:204–205

    CAS  PubMed  Google Scholar 

  • Yue GH, Chang A (2010) Molecular evidence for high frequency of multiple paternity in a freshwater shrimp species Caridina ensifera. PLoS One 5:e12721

    PubMed  PubMed Central  Google Scholar 

  • Yue X, Nie Q, Xiao G, Liu B (2015) Transcriptome analysis of shell color-related genes in the clam Meretrix meretrix. Mar Biotechnol 17:364–374

    CAS  Google Scholar 

  • Zeng HS, Ding SX, Wang J, Su YQ (2008) Characterization of eight polymorphic microsatellite loci for the giant grouper (Epinephelus lanceolatus Bloch). Mol Ecol Resour 8:805–807

    CAS  PubMed  Google Scholar 

  • Zhang J, Ma W, Song X, Lin Q, Gui JF, Mei J (2014) Characterization and development of EST-SSR markers derived from transcriptome of yellow catfish. Molecules 19:16402–16415

    PubMed  PubMed Central  Google Scholar 

  • Zhao Y, Zhu X, Li Z, Xu W, Dong J, Wei H, Li Y, Li X (2019) Genetic diversity and structure of Chinese grass shrimp, Palaemonetes sinensis, inferred from transcriptome-derived microsatellite markers. BMC Genet 20:75

    PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgments

We are very grateful to Prof. CH Liou and YS Huang for their helpful comments on this work. We thank the members of the aquaculture team of our department for taking care of our ornamental shrimp breeding stocks.

Funding

This research is supported by the Fisheries Agency, Council of Agriculture, Taiwan, with grant number 104AS-16.2.6-FA-F1.

Author information

Authors and Affiliations

  1. Department of Aquaculture, National Taiwan Ocean University, 2 Beining Road, Jhongjheng District, Keelung City, 20224, Taiwan

    Chang-Wen Huang, Pei-Yun Chu, Yu-Fang Wu & Wei-Ren Chan

  2. Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, Taiwan

    Chang-Wen Huang

  3. Larmax International Co., Ltd. No.9, Yuanxi 2nd Rd., Changzhi, Pingtung, Taiwan

    Yeh-Hao Wang

Authors
  1. Chang-Wen Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pei-Yun Chu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yu-Fang Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wei-Ren Chan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yeh-Hao Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

C.W.H. was involved in planning and supervised the project. P.Y.C. and Y.F.W. contributed to the design and implementation of the research, to the analysis of the results, and to the writing of the manuscript. P.Y.C., Y.F.W., and W.R.C. processed the experimental data and performed the analysis. W.R.C. and Y.H.W. manufactured the samples. C.W.H., P.Y.C., Y.F.W., W.R.C., and Y.H.W. discussed the results and commented on the manuscript. C.W.H. finalized the paper.

Corresponding author

Correspondence to Chang-Wen Huang.

Ethics declarations

Competing Interests

The authors declare that they have no competing interests.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic Supplementary Material

ESM 1

(DOCX 210 kb)

ESM 2

(DOCX 264 kb)

ESM 3

(DOCX 310 kb)

ESM 4

(DOCX 276 kb)

ESM 5

(XLSX 10.8 kb)

ESM 6

(XLSX 10.1 kb)

ESM 7

(XLSX 9.81 kb)

ESM 8

(XLSX 10.5 kb)

ESM 9

(XLSX 10.4 kb)

ESM 10

(XLSX 11.2 kb)

ESM 11

(XLSX 12.2 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Huang, CW., Chu, PY., Wu, YF. et al. Identification of Functional SSR Markers in Freshwater Ornamental Shrimps Neocaridina denticulata Using Transcriptome Sequencing. Mar Biotechnol 22, 772–785 (2020). https://doi.org/10.1007/s10126-020-09979-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10126-020-09979-y

Keywords

Search

Navigation