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Construction of High-Density Genetic Map and Mapping of Sex-Related Loci in the Yellow Catfish (Pelteobagrus fulvidraco)

Marine Biotechnology volume 22, pages 31–40 (2020)Cite this article

Abstract

The yellow catfish (Pelteobagrus fulvidraco) is a very important aquaculture species distributed in freshwater area of China. All-male yellow catfish is very popular in aquaculture because of their significant sex dimorphism phenomena. The males grow much faster than females in full-sibling family. However, the sex dimorphism mechanism is still unclear in yellow catfish. In order to better understand the genetic basis of yellow catfish sexual dimorphism, it is vital to map the sex-related traits and localize the candidate genes across yellow catfish whole genome. Here, we constructed a high-density linkage map of yellow catfish using genotyping-by-sequencing (GBS) strategy. A total of 5705 single-nucleotide polymorphism (SNP) markers were mapped to 26 different linkage groups (LGs) using 184 F1 offspring. The total genetic map length was 3071.59 cM, with an average interlocus distance of 0.54 cM. Eleven significant sex-related QTLs in yellow catfish were identified. Six sex-related genes were identified from the region of reference genome near these QTLs including amh, gnrhr, vasa, lnnr1, foxl2, and bmp15. The high-density genetic linkage map provides valuable resources for yellow catfish molecular assistant breeding and elucidating sex differentiation process. Moreover, the comparative genomic study was analyzed among yellow catfish, channel catfish, and zebrafish. It revealed highly conserved chromosomal distribution between yellow catfish and channel catfish.

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Funding

This research was supported by grants from Scientific Technology Plan Program of Guangzhou City (201803020017).

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Affiliations

  1. School of Marine Sciences, Sun Yat-sen University, Guangzhou, China

    Dong Gao, Min Zheng, Genmei Lin, Wenyu Fang, Jing Huang & Jianguo Lu

  2. Southern Marine Science and Engineering Guangdong Laboratory, Zhuhai, China

    Min Zheng & Jianguo Lu

  3. Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, China

    Xiaowen Sun

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  1. Dong Gao
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  2. Min Zheng
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  3. Genmei Lin
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  4. Wenyu Fang
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  6. Jianguo Lu
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Correspondence to Min Zheng or Jianguo Lu.

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ESM 1

Additional file 1: Table S1 Summary of trimmed reads from 186 individuals aligned to unique location of the reference sequence. Additional file 2: Table S2 Information of LMD40 SNP markers. Additional file 3: Table S3 Tabular representation of the integrated map of yellow catfish. Additional file 4: Table S4 Tabular representation of the maternal map of yellow catfish. Additional file 5: Fig. S1 Linkage group lengths and maker distributions of pBCM genetic map of the yellow catfish. Additional file 6: Table S5 Tabular representation of the paternal map of yellow catfish. Additional file 7: Fig. S2 Linkage group lengths and maker distributions of pF2 genetic map of the yellow catfish (RAR 3107 kb)

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Gao, D., Zheng, M., Lin, G. et al. Construction of High-Density Genetic Map and Mapping of Sex-Related Loci in the Yellow Catfish (Pelteobagrus fulvidraco). Mar Biotechnol 22, 31–40 (2020). https://doi.org/10.1007/s10126-019-09928-4

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Keywords

  • Yellow catfish
  • Genetic linkage map
  • Sex-related genes
  • Quantitative trait locus