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MicroRNAs Involved in the Regulation of LC-PUFA Biosynthesis in Teleosts: miR-33 Enhances LC-PUFA Biosynthesis in Siganus canaliculatus by Targeting insig1 which in Turn Upregulates srebp1

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Abstract

Post-transcriptional regulatory mechanisms play important roles in the regulation of LC-PUFA biosynthesis. Our previous study revealed that miR-33 could increase the expression of fatty acyl desaturases (fads2) in the rabbitfish Siganus canaliculatus, but the specific mechanism is unknown. Here, we confirmed that miR-33 could target the 3′UTR of insulin-induced gene 1 (insig1), resulting in downregulation of its protein level in the rabbitfish hepatocyte line (SCHL). In vitro overexpression of miR-33 inhibited the mRNA level of insig1 and increased the mRNA levels of Δ6Δ5 fads2 and elovl5, as well as srebp1. In SCHL cells, proteolytic activation of sterol-regulatory-element-binding protein-1 (Srebp1) was blocked by Insig1, with overexpression of insig1 decreasing mature Srebp1 level, while inhibition of insig1 led to the opposite effect. Srebp1 could enhance the promoter activity of Δ6Δ5 fads2 and elovl5, whose expression levels decreased with knockdown of srebp1 in SCHL. Overexpression of miR-33 also resulted in a higher conversion of 18:3n-3 to 18:4n-3 and 20:5n-3 to 22:5n-3, linked to desaturation and elongation via Δ6Δ5 Fads2 and Elovl5, respectively. The results suggested that the mechanism by which miR-33 regulates LC-PUFA biosynthesis in rabbitfish is through enhancing the expression of srebp1 by targeting insig1. The findings here provide more insight to the mechanism of miRNAs involvement in the regulation of LC-PUFA biosynthesis in teleosts.

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Funding

This work was financially supported by the National Key R&D Program of China (2018YFD0900400), National Natural Science Foundation of China (No. 31873040 and No. 31702357), Natural Science Foundation of Guangdong Province (2018A030313910), China Agriculture Research System (CARS-47) and Guangdong Agriculture Research System (Freshwater Fish).

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Authors and Affiliations

  1. Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, 515063, China

    Jun Jun Sun, Li Guo Zheng, Cui Ying Chen, Jin Ying Zhang, Cui Hong You, Qing Hao Zhang, Hong Yu Ma & Shu Qi Wang

  2. School of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China

    Yuan You Li

  3. Instituto de Acuicultura Torre de la Sal, Consejo Superior de Investigaciones Científicas (IATS-CSIC), 12595 Ribera de Cabanes, Castellón, Spain

    Óscar Monroig

  4. Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, Stirling, Scotland, FK9 4LA, UK

    Douglas R. Tocher

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  1. Jun Jun Sun
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Correspondence to Shu Qi Wang or Yuan You Li.

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Sun, J.J., Zheng, L.G., Chen, C.Y. et al. MicroRNAs Involved in the Regulation of LC-PUFA Biosynthesis in Teleosts: miR-33 Enhances LC-PUFA Biosynthesis in Siganus canaliculatus by Targeting insig1 which in Turn Upregulates srebp1. Mar Biotechnol 21, 475–487 (2019). https://doi.org/10.1007/s10126-019-09895-w

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