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Carrageenans from Sarcothalia crispata and Gigartina skottsbergii: Structural Analysis and Interpolyelectrolyte Complex Formation for Drug Controlled Release

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Abstract

The aims of the present study were to characterize for the first time the carrageenan extracted from cystocarpic stage of S. crispata collected in the Patagonian coast of Argentina, and to prepare interpolyelectrolytic complexes (IPECs) between the polysaccharide extracted from cystocarpic stage of Sarcothalia crispata and Gigartina skottsbergii thalli, and basic butylated methacrylate copolymer (Eudragit E), in order to test their potential for the controlled release of ibuprofen as model drug. The structural determination revealed that the polysaccharides extracted from S. crispata and G. skottsbergii were mainly constituted by κ-carrageenan, particularly in the case of G. skottsbergii; however, significant amounts of ι- and ν-carrageenan were also detected in both polygalactans. The differences in diad composition and possibly in their distribution along the polysaccharide chain of both carrageenans would favor a different arrangement in the resulting IPEC structure. The smaller pores observed by scanning electron microscopy in the IPEC of S. crispata suggest that the kinks in the polysaccharide backbone are evenly distributed, resulting in a slower ibuprofen release compared to the IPEC of G. skottsbergii.

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Acknowledgements

The authors are indebted to Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina (CONICET) PIP 112-201101-00208 and 112-2015 01-00510, Secretaría de Ciencia y Tecnología de la Universidad Nacional del Sur, PGI 24/B246 and Secretaría de Ciencia y Técnica, Universidad de Buenos Aires, UBACyT 20020130100216BA. MHH is a Fellow Member of CONICET. HJP, PIL, and MCM are Research Members of CONICET.

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Authors and Affiliations

  1. Centro de Recursos Naturales Renovables de la Zona Semiárida (CERZOS) CONICET-UNS, Camino La Carrindanga Km 7, 8000, Bahía Blanca, Argentina

    Melanie Hebe Hughes, Karina Michetti & Patricia Inés Leonardi

  2. Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur, San Juan 670, 8000, Bahía Blanca, Argentina

    Melanie Hebe Hughes, Karina Michetti & Patricia Inés Leonardi

  3. Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, PINMATE-Departamento de Industrias, Ciudad Universitaria, 1428EGA, Buenos Aires, Argentina

    Héctor Juan Prado

  4. Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Departamento de Tecnología Farmacéutica II, Junín 956, 1113AAD, Buenos Aires, Argentina

    Héctor Juan Prado

  5. Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Biodiversidad y Biología Experimental, Ciudad Universitaria, 1428EGA, Buenos Aires, Argentina

    María Cecilia Rodríguez

  6. Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Centro de Investigación en Hidratos de Carbono (CIHIDECAR), Facultad de Ciencias Exactas y Naturales,Departamento de Química Orgánica, Ciudad Universitaria, 1428EGA, Buenos Aires, Argentina

    María Cecilia Rodríguez & María Cristina Matulewicz

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  1. Melanie Hebe Hughes
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  2. Héctor Juan Prado
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  3. María Cecilia Rodríguez
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  4. Karina Michetti
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  6. María Cristina Matulewicz
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Correspondence to María Cristina Matulewicz.

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Fig. S1

FTIR of the carrageenans from S. crispata (SC), G. skottsbergii (GS), Eudragit E (EE) and the corresponding IPECs (PNG 237 kb)

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Hughes, M.H., Prado, H.J., Rodríguez, M.C. et al. Carrageenans from Sarcothalia crispata and Gigartina skottsbergii: Structural Analysis and Interpolyelectrolyte Complex Formation for Drug Controlled Release. Mar Biotechnol 20, 706–717 (2018). https://doi.org/10.1007/s10126-018-9842-4

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