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Identification of a Sorbicillinoid-Producing Aspergillus Strain with Antimicrobial Activity Against Staphylococcus aureus: a New Polyextremophilic Marine Fungus from Barents Sea

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Abstract

The exploration of poorly studied areas of Earth can highly increase the possibility to discover novel bioactive compounds. In this study, the cultivable fraction of fungi and bacteria from Barents Sea sediments has been studied to mine new bioactive molecules with antibacterial activity against a panel of human pathogens. We isolated diverse strains of psychrophilic and halophilic bacteria and fungi from a collection of nine samples from sea sediment. Following a full bioassay-guided approach, we isolated a new promising polyextremophilic marine fungus strain 8Na, identified as Aspergillus protuberus MUT 3638, possessing the potential to produce antimicrobial agents. This fungus, isolated from cold seawater, was able to grow in a wide range of salinity, pH and temperatures. The growth conditions were optimised and scaled to fermentation, and its produced extract was subjected to chemical analysis. The active component was identified as bisvertinolone, a member of sorbicillonoid family that was found to display significant activity against Staphylococcus aureus with a minimum inhibitory concentration (MIC) of 30 μg/mL.

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Funding

This work was supported by the EU FP7 KBBE2012-2016 project: “Pharmasea,” Grant Agreement 312184, and by the H2020-MSCA-RISE: Ocean Medicines, Grant Agreement 690944.

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Author notes

  1. Paulina Corral, Fortunato Palma Esposito and Pietro Tedesco contributed equally to this work.

Authors and Affiliations

  1. National Research Council of Italy (CNR)-Institute of Protein Biochemistry (IBP), Naples, Italy

    Paulina Corral, Fortunato Palma Esposito, Pietro Tedesco, Angela Falco, Emiliana Tortorella & Donatella de Pascale

  2. Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL, USA

    Paulina Corral

  3. Department of Pharmacy, University of Naples Federico II, Naples, Italy

    Luciana Tartaglione, Carmen Festa & Maria Valeria D’Auria

  4. Department of Life Science and Systems Biology, University of Torino, Turin, Italy

    Giorgio Gnavi & Giovanna Cristina Varese

Authors
  1. Paulina Corral
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  2. Fortunato Palma Esposito
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  3. Pietro Tedesco
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  4. Angela Falco
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  5. Emiliana Tortorella
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  6. Luciana Tartaglione
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  7. Carmen Festa
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  8. Maria Valeria D’Auria
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  9. Giorgio Gnavi
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  10. Giovanna Cristina Varese
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  11. Donatella de Pascale
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Contributions

P.C., F.P.E. and P.T. equally contributed to the work. P.C., F.P.E, A.F. and D.d.P. conceived and designed the experiments; P.C., F.P.E., P.T., A.F., E.T., L.T., M.V.D.A., G.G. and G.C.V. performed the experiments; P.C., F.P.E, P.T., A.F. and D.d.P. analysed the data; G.C.V., L.T., M.V.D.A. and D.d.P. contributed reagent/material/analysis tools; P.C., F.P.E., P.T. and D.d.P. wrote the paper.

Corresponding author

Correspondence to Donatella de Pascale.

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The authors declare that they have no conflict of interest.

Electronic Supplementary Material

The following are available online at, Figure S1: Cluster analysis of the DNA fingerprinting profiles generated from genomic DNA of Aspergillus spp. (A) and Penicillum spp.(B) isolates, using the microsatellite primer M-13; Figure S2: Qualitative interpretation of antimicrobial screening by Cross Streaking; Figure S3: HRESI MS spectrum (positive ion mode) of peak 9; Figure S4: 1H NMR spectrum (CDCl3) of peak 9; Figure S5: High resolution MS/MS spectrum of the precursor ion at m/z 513.2.

Figure S1

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Corral, P., Esposito, F.P., Tedesco, P. et al. Identification of a Sorbicillinoid-Producing Aspergillus Strain with Antimicrobial Activity Against Staphylococcus aureus: a New Polyextremophilic Marine Fungus from Barents Sea. Mar Biotechnol 20, 502–511 (2018). https://doi.org/10.1007/s10126-018-9821-9

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  • DOI: https://doi.org/10.1007/s10126-018-9821-9

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