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Genome Sequence of Microbulbifer mangrovi DD-13T Reveals Its Versatility to Degrade Multiple Polysaccharides

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Abstract

Microbulbifer mangrovi strain DD-13T is a novel-type species isolated from the mangroves of Goa, India. The draft genome sequence of strain DD-13 comprised 4,528,106 bp with G+C content of 57.15%. Out of 3479 open reading frames, functions for 3488 protein coding sequences were predicted on the basis of similarity with the cluster of orthologous groups. In addition to protein coding sequences, 34 tRNA genes and 3 rRNA genes were detected. Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs). Many genes from strain DD-13 were annotated as carbohydrases specific for degradation of agar, alginate, carrageenan, chitin, xylan, pullulan, cellulose, starch, β-glucan, pectin, etc. Some of polysaccharide-degrading genes were highly modular and were appended at least with one CBM indicating the versatility of strain DD-13 to degrade complex polysaccharides. The cell growth of strain DD-13 was validated using pure polysaccharides such as agarose or alginate as carbon source as well as by using red and brown seaweed powder as substrate. The homologous carbohydrase produced by strain DD-13 during growth degraded the polysaccharide, ensuring the production of metabolizable reducing sugars. Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.

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Acknowledgment

Md. Imran acknowledges the Department of Biotechnology, New Delhi, Govt. of India, for the financial support as JRF and SRF throughout the tenure of this work.

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Authors and Affiliations

  1. Department of Biotechnology, Goa University, Taleigao Plateau, Goa, 403206, India

    Md. Imran, Yogini P. Shanbhag & Sanjeev C. Ghadi

  2. Plant Molecular Biology Laboratory, CSIR-National Botanical Research Institute, Lucknow, 226001, India

    Poonam Pant & Samir V. Sawant

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  1. Md. Imran
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  2. Poonam Pant
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  3. Yogini P. Shanbhag
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  4. Samir V. Sawant
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  5. Sanjeev C. Ghadi
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Corresponding author

Correspondence to Sanjeev C. Ghadi.

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Accession No.

This Whole Genome Shotgun project has been deposited at DDBJ/ENA/GenBankunder the accession LZDE00000000. The version described in this paper is version LZDE01000000.

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Imran, M., Pant, P., Shanbhag, Y.P. et al. Genome Sequence of Microbulbifer mangrovi DD-13T Reveals Its Versatility to Degrade Multiple Polysaccharides. Mar Biotechnol 19, 116–124 (2017). https://doi.org/10.1007/s10126-017-9737-9

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  • DOI: https://doi.org/10.1007/s10126-017-9737-9

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