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Marine Biotechnology

, Volume 16, Issue 4, pp 371–384 | Cite as

Rubisco Expression in the Dinoflagellate Symbiodinium sp. Is Influenced by Both Photoperiod and Endosymbiotic Lifestyle

  • Anderson B. Mayfield
  • Yi-Yuong Hsiao
  • Hung-Kai Chen
  • Chii-Shiarng ChenEmail author
Original Article

Abstract

Although the importance of anthozoan-dinoflagellate (genus Symbiodinium) endosymbioses in the establishment of coral reef ecosystems is evident, little is known about the molecular regulation of photosynthesis in the intra-gastrodermal symbiont communities, particularly with respect to the rate-limiting Calvin cycle enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco). In this study, we analyzed rubisco mRNA (rbcL) and protein (RBCL) concentrations over the diel cycle in both cultured and endosymbiotic Symbiodinium samples. In the former, rbcL expression increased upon illumination and decreased during the dark, a pattern that was upheld under continual dark incubation. A different trend in rbcL expression was observed in endosymbiotic Symbiodinium residing within sea anemone (Aiptasia pulchella) tissues, in which illumination gradually led to decreased rbcL mRNA expression. Unexpectedly, RBCL protein expression did not vary over time within anemone tissues, and in neither cultured nor endosymbiotic samples was a correlation between gene and protein expression documented. It appears, then, that photoperiod, lifestyle, and posttranscriptional regulation are all important drivers of RBCL expression in this ecologically important dinoflagellate.

Keywords

Cnidarian Dinoflagellate Endosymbiosis Photosynthesis Rubisco Symbiodinium 

Notes

Acknowledgments

ABM was funded by an international research fellowship from the United States National Science Foundation (OCE-0852960) and the Khaled bin Sultan Living Oceans Foundation. NMMBA and National Science Council (NSC 98-2311-B-291-001-MY3 and 101-2311-B-291-002-MY3) provided funds to CSC that were instrumental to the success of the laboratory analyses.

Supplementary material

10126_2014_9558_MOESM1_ESM.jpg (192 kb)
Fig. s1 Primer specificity validation study. (A) Symbiodinium density in Aiptasia pulchella specimens of different symbiotic status (healthy, partially bleached, and fully bleached) was observed with DIC microscopy. (B) The same samples as in (A) were also imaged under a fluorescent microscope. The red fluorescence is from the chlorophyll of the Symbiodinium populations while the green represents autofluorescence of the host. (C) Selected insets in (B) were magnified to better demonstrate Symbiodinium density in hospite. (D) Neither rbcL nor act1 mRNAs were detected in partially and fully bleached specimens after 27 PCR cycles. In partially bleached A. pulchella, only the act1 mRNA could be detected after 40 cycles. (JPEG 191 kb)
10126_2014_9558_MOESM2_ESM.ai (1 mb)
Fig. s2 RFLPs. A portion of the Symbiodinium 18s rDNA gene was amplified with PCR, digested with either TaqI or Sau3 AI, and electrophoresed as described in the text. Two clade B Symbiodinium samples from a previous study (Wang et al. 2008) are shown as a reference (A), and four representative samples are shown for each of the following groups: the Symbiodinium cultures (B), anemones of the 12L:12D study (C), and anemones of the primer specificity validation study (PSVS; D). In all panels, lanes 1 and 4, and, when applicable, 7 and 10, represent the undigested, ~1,500 bp PCR product. Lanes 2 and 5, and, when applicable, 8 and 11, represent TaqI-digested amplicons. Lanes 3 and 6, and, when applicable, 9 and 12, represent Sau3 AI-digested amplicons. DNA ladders are shown in each panel. (AI 1025 kb)

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Anderson B. Mayfield
    • 1
    • 2
  • Yi-Yuong Hsiao
    • 1
    • 3
  • Hung-Kai Chen
    • 1
    • 3
    • 4
  • Chii-Shiarng Chen
    • 1
    • 3
    • 4
    Email author
  1. 1.Taiwan Coral Research Center (TCRC)National Museum of Marine Biology and AquariumPingtung 944Republic of China
  2. 2.Living Oceans FoundationLandoverUSA
  3. 3.Graduate Institute of Marine BiotechnologyNational Dong-Hwa UniversityPingtungRepublic of China
  4. 4.Department of Marine Biotechnology and ResourcesNational Sun Yat-Sen UniversityKaohsiungRepublic of China

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