Marine Biotechnology

, Volume 16, Issue 3, pp 309–320 | Cite as

Intraperitoneal Germ Cell Transplantation in the Nile Tilapia Oreochromis niloticus

  • Rodolfo Farlora
  • Shoko Hattori-Ihara
  • Yukata Takeuchi
  • Makoto Hayashi
  • Anna Octavera
  • Alimuddin
  • Goro YoshizakiEmail author
Original Article


Germ cell transplantation offers promising applications in finfish aquaculture and the preservation of endangered species. Here, we describe an intraperitoneal spermatogonia transplantation procedure in the Nile tilapia Oreochromis niloticus. Through histological analysis of early gonad development, we first determined the best suitable stage at which exogenous germ cells should be transplanted into the recipients. For the transplantation procedure, donor testes from a transgenic Nile tilapia strain carrying the medaka β-actin/enhanced green fluorescent protein (EGFP) gene were subjected to enzymatic dissociation. These testicular cells were then stained with PKH26 and microinjected into the peritoneal cavity of the recipient fish. To confirm colonization of the donor-derived germ cells, the recipient gonads were examined by fluorescent and confocal microscopy. PKH26-labeled cells exhibiting typical spermatogonial morphology were incorporated into the recipient gonads and were not rejected within 22 days posttransplantation. Long-term survival of transgenic donor-derived germ cells was then verified in the gonads of 5-month-old recipients and in the milt and vitelogenic oocytes of 1-year-old recipients, by means of PCR using EGFP-specific primers. EGFP-positive milt from adult male recipients was used to fertilize non-transgenic oocytes and produced transgenic offspring expressing the donor-derived phenotype. These results imply that long-term survival, proliferation, and differentiation of the donor-derived spermatogonia into vitelogenic oocytes and functional spermatozoa are all possible. Upon further improvements in the transplantation efficiency, this intraperitoneal transplantation system could become a valuable tool in the conservation of genetic resources for cichlid species.


Germ cell transplantation Spermatogonia Oreochromis niloticus Endangered species Transgenic fish Green fluorescent protein (GFP) 



This research was partly supported by the Japan Science and Technology Agency/Japan International Cooperation Agency through their Science and Technology Research Partnership for Sustainable Development program to GY.


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Rodolfo Farlora
    • 1
  • Shoko Hattori-Ihara
    • 2
  • Yukata Takeuchi
    • 3
  • Makoto Hayashi
    • 2
  • Anna Octavera
    • 4
  • Alimuddin
    • 4
  • Goro Yoshizaki
    • 2
    • 5
    Email author
  1. 1.Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research (INCAR)University of ConcepcionConcepciónChile
  2. 2.Department of Marine BiosciencesTokyo University of Marine Science and TechnologyTokyoJapan
  3. 3.Research Center for Advanced Science and TechnologyTokyo University of Marine Science and TechnologyChibaJapan
  4. 4.Department of Aquaculture, Faculty of Fisheries and Marine SciencesBogor Agriculture UniversityBogorIndonesia
  5. 5.Japan Science and Technology Agency/Japan International Cooperation Agency (JST/JICA) through their Science and Technology Research Partnership for Sustainable Development (SATREPS)ChibaJapan

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