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Identification of Quantitative Trait Loci Associated with Resistance to Viral Haemorrhagic Septicaemia (VHS) in Turbot (Scophthalmus maximus): A Comparison Between Bacterium, Parasite and Virus Diseases

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Abstract

One of the main objectives of genetic breeding programs in turbot industry is to reduce disease-related mortality. In the present study, a genome scan to detect quantitative trait loci (QTL) affecting resistance and survival to viral haemorrhagic septicaemia (VHS) was carried out. Three full-sib families with approximately 90 individuals each were genotyped and evaluated by linear regression and maximum likelihood approaches. In addition, a comparison between QTL detected for resistance and survival time to other important bacterial and parasite diseases affecting turbot (furunculosis and scuticociliatosis) was also carried out. Finally, the relationship between QTL affecting resistance/survival time to the virus and growth-related QTL was also evaluated. Several genomic regions controlling resistance and survival time to VHS were detected. Also significant associations between the evaluated traits and genotypes at particular markers were identified, explaining up to 14 % of the phenotypic variance. Several genomic regions controlling general and specific resistance to different diseases in turbot were detected. A preliminary gene mining approach identified candidate genes related to general or specific immunity. This information will be valuable to develop marker-assisted selection programs and to discover candidate genes related to disease resistance to improve turbot production.

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Acknowledgements

This study was supported by the Spanish government project (Consolider Ingenio Aquagenomics: CSD200700002).

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Authors and Affiliations

  1. Departamento de Bioquímica, Genética e Inmunología, Facultad de Biología, Universidad de Vigo, 36310, Vigo, Spain

    Silvia T. Rodríguez-Ramilo

  2. Departamento de Mejora Genética Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Ctra. A Coruña Km. 7.5, 28040, Madrid, Spain

    Silvia T. Rodríguez-Ramilo & Jesús Fernández

  3. Departamento de Genética, Facultad de Ciencias, Universidad de Granada, Avenida Fuentenueva s/n, 18071, Granada, Spain

    Roberto De La Herrán & Carmelo Ruiz-Rejón

  4. Departamento de Genética, Facultad de Veterinaria, Universidad de Santiago de Compostela, Campus de Lugo, 27002, Lugo, Spain

    Miguel Hermida, Carlos Fernández, Carmen Bouza & Paulino Martínez

  5. Instituto de Investigaciones Marinas, CSIC, Eduardo Cabello 6, 36208, Vigo, Spain

    Patricia Pereiro & Antonio Figueras

  6. Departamento de Producción Animal, ETS Ingenieros Agrónomos, Universidad Politécnica de Madrid, Ciudad Universitaria, 28040, Madrid, Spain

    Miguel A. Toro

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  1. Silvia T. Rodríguez-Ramilo
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  2. Roberto De La Herrán
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  9. Miguel A. Toro
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  10. Paulino Martínez
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Correspondence to Jesús Fernández.

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Rodríguez-Ramilo, S.T., De La Herrán, R., Ruiz-Rejón, C. et al. Identification of Quantitative Trait Loci Associated with Resistance to Viral Haemorrhagic Septicaemia (VHS) in Turbot (Scophthalmus maximus): A Comparison Between Bacterium, Parasite and Virus Diseases. Mar Biotechnol 16, 265–276 (2014). https://doi.org/10.1007/s10126-013-9544-x

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