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Transcriptomic Response of Skeletal Muscle to Lipopolysaccharide in the Gilthead Seabream (Sparus aurata)

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Abstract

The physiological consequences of the activation of the immune system in fish are not well understood. In particular, skeletal muscle, due to its essential role in locomotion and whole-animal energy homeostasis, is a potentially important target of inflammation. In this study, we have evaluated the in vivo effects of lipopolysaccharide (LPS) on the white and red skeletal muscle transcriptome of the gilthead seabream (Sparus aurata) by microarray analysis at 24 and 72 h after injection. In white muscle, the transcriptomic response was characterized by an up-regulation of genes involved in carbohydrate catabolism and protein synthesis at 24 h and a complete reversal of this pattern at 72 h. In red muscle, an up-regulation of genes involved in carbohydrate catabolism and protein synthesis was observed only at 72 h after LPS administration. Interestingly, both white and red muscles showed a similar consistent down-regulation of immune genes at 72 h post-injection. However, genes involved in muscle contraction showed a general up-regulation in response to LPS in both types of muscle. In summary, LPS administration causes muscle type-specific responses regarding the expression of genes involved in carbohydrate and protein metabolism and a common decreased expression of immune genes in skeletal muscle, concomitant with increased expression of genes for contractile elements. Our results evidence a robust and tissue-specific transcriptomic response of the skeletal muscle to an acute inflammatory challenge.

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Acknowledgments

This research has been funded by grants CSD2007-0002 and HG2005-0008 from the Spanish Ministry of Science and Innovation to JVP and by GSRT of the Ministry of Development under the Research and Technology Cooperation scheme between Greece and Spain 2005-2007 to EA. We would also like to thank the Turku Centre of Biotechnology (Finland) for the preparation of microarrays and Dr. Aleksei Krasnov (Nofima Marin) for his assistance with microarray analysis.

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Authors and Affiliations

  1. Department of Zoology, School of Biology, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece

    Elisavet Kaitetzidou & Efthimia Antonopoulou

  2. Departament de Fisiologia i Immunologia, Facultat de Biologia, Universitat de Barcelona, 08028, Barcelona, Spain

    Diego Crespo, Yoryia Vraskou & Josep V. Planas

  3. Institut de Biomedicina de la Universitat de Barcelona (IBUB), 08028, Barcelona, Spain

    Josep V. Planas

Authors
  1. Elisavet Kaitetzidou
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  2. Diego Crespo
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  3. Yoryia Vraskou
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  4. Efthimia Antonopoulou
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  5. Josep V. Planas
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Corresponding author

Correspondence to Josep V. Planas.

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Elisavet Kaitetzidou and Diego Crespo contributed equally to this study.

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ESM 1

Complete list of differentially expressed genes in white muscle at 24 h after lipopolysaccharide administration. Only significantly up- and down-regulated genes (p < 0.01, Student’s t-test, 12 spot replicates per gene) are shown (XLSX 22 kb)

ESM 2

Complete list of differentially expressed genes in white muscle at 72 h after lipopolysaccharide administration. Only significantly up- and down-regulated genes (p < 0.01, Student’s t-test, 12 spot replicates per gene) are shown (XLSX 26 kb)

ESM 3

Complete list of differentially expressed genes in red muscle at 24 h after lipopolysaccharide administration. Only significantly up- and down-regulated genes (p < 0.01, Student’s t-test, 12 spot replicates per gene) are shown (XLSX 13 kb)

ESM 4

Complete list of differentially expressed genes in red muscle at 72 h after lipopolysaccharide administration. Only significantly up- and down-regulated genes (p < 0.01, Student’s t-test, 12 spot replicates per gene) are shown (XLSX 22 kb)

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Kaitetzidou, E., Crespo, D., Vraskou, Y. et al. Transcriptomic Response of Skeletal Muscle to Lipopolysaccharide in the Gilthead Seabream (Sparus aurata). Mar Biotechnol 14, 605–619 (2012). https://doi.org/10.1007/s10126-012-9469-9

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  • DOI: https://doi.org/10.1007/s10126-012-9469-9

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