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Mannan-Binding Lectin of the Sea Urchin Strongylocentrotus nudus

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Abstract

A novel lectin specific to low-branched mannans (MBL-SN) was isolated from coelomic plasma of the sea urchin Strongylocentrotus nudus by combining anion-exchange liquid chromatography on DEAE Toyopearl 650 M, affinity chromatography on mannan-Sepharose and gel filtration on the Sephacryl S-200. The molecular mass of MBL-SN was estimated by sodium dodecyl sulphate polyacrylamide gel electrophoresis under non-reducing conditions to be about 34 kDa. MBL-SN was shown to be a dimer with two identical subunits of about 17 kDa. The native MBL-SN exists as a tetramer. The physico-chemical properties of MBL-SN indicate that it belongs to C-type mannan-binding lectins. The cDNA encoding MBL-SN was cloned from the total cDNA of S. nudus coelomocytes and encodes a 17-kDa protein of 144 amino acid residues that contains a single carbohydrate-recognition domain of C-type lectins. Prediction of the MBL-SN tertiary structure using comparative modelling revealed that MBL-SN is an α/β-protein with eight β-strands and two α-helices. Comparison of the MBL-SN model with available three-dimensional structures of C-type lectins revealed that they share a common fold pattern.

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Abbreviations

CEA:

Carcinoembryonic antigen

CRD:

Carbohydrate-recognition domain

CTL:

C-type lectin

EDTA:

Ethylenediaminetetraacetic acid

HEPES:

N-2-Hydroxyethylpiperazine-N-[2-ethanesulfonic] acid

MBL-AJ:

Mannan-binding lectins from Apostichopus japonicus

MBL-CJ:

Mannan-binding lectins from Cucumaria japonica

MBL-SN:

Mannan-binding lectins from Strongylocentrotus nudus

MBLs:

Mannan-binding lectins

MES:

2-[N-Morpholino]ethanesulfonic acid

TB-Ca2+ :

Tris buffer, containing 10 mM CaCl2 (0.01 M Tris–HCl, pH 7.5, 10 mM CaCl2)

TBS-Ca2+ :

Tris-buffered saline, containing 10 mM CaCl2 (0.01 M Tris–HCl, pH 7.5, 0.15 M NaCl and 10 mM CaCl2)

TBS-T:

Tris-buffered saline, containing 0.05 % Triton X-100

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Acknowledgments

We are thankful to Dr. Evgeniy L. Nazarenko (PIBOC, Russia) for the kindly loaned samples of mannans from marine bacteria.

Funding

This work was supported by the Russian Foundation for Basic Research (grant numbers 11-04-01778-a); the Eastern Branch Russian Academy of Sciences (09-III-A-05-150) and Russian Federation Government were supporting the scientific research conducted by leading scientists on the base of Russian Higher Education Institutions (contract no. 11.G34.31.0010). FEBRAS grant no. 12-III-A-05-059, and the Program of the Russian Academy of Science Presidium ‘‘Molecular and Cellular Biology’’ (grant no.12-I-P6-10).

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Authors and Affiliations

  1. G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Science, Stoletya Vladivostoku str. 159, Vladivostok, 690022, Russia

    Aleksandr A. Bulgakov, Svetlana N. Kovalchuk, Galina N. Likhatskaya & Valery A. Rasskazov

  2. A.V. Zhirmunsky Institute of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences, Palchevskogo str. 17, Vladivostok, 690059, Russia

    Marina G. Eliseikina, Irina Yu Petrova & Ekaterina V. Shamshurina

  3. Interdepartmental Laboratory “Biology of Marine Invertebrates”, Far Eastern Federal University, 8, Sukhanova str., Vladivostok, 690950, Russia

    Ekaterina V. Shamshurina

  4. Division of Cell Biology and Genetics, Far Eastern Federal University, 8, Sukhanova str., Vladivostok, 690950, Russia

    Svetlana N. Kovalchuk

Authors
  1. Aleksandr A. Bulgakov
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  2. Marina G. Eliseikina
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  3. Svetlana N. Kovalchuk
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  4. Irina Yu Petrova
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Correspondence to Aleksandr A. Bulgakov.

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Bulgakov, A.A., Eliseikina, M.G., Kovalchuk, S.N. et al. Mannan-Binding Lectin of the Sea Urchin Strongylocentrotus nudus . Mar Biotechnol 15, 73–86 (2013). https://doi.org/10.1007/s10126-012-9460-5

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