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Genetic Positioning of Centromeres through Half-Tetrad Analysis in Gynogenetic Diploid Families of the Zhikong Scallop (Chlamys farreri)

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Abstract

Centromere mapping is a powerful tool for improving linkage maps, investigating crossover events, and understanding chiasma interference during meiosis. Ninety microsatellite markers selected across all linkage groups (LGs) from a previous Chlamys farreri genetic map were studied in three artificially induced meiogynogenetic families for centromere mapping by half-tetrad analysis. Inheritance analyses showed that all 90 microsatellite loci conformed to Mendelian inheritance in the control crosses, while 4.4 % of the microsatellite loci showed segregation departures from an expected 1:1 ratio of two homozygote classes in meiogynogenetic progeny. The second division segregation frequency (y) of the microsatellites ranged from 0.033 to 0.778 with a mean of 0.332, confirming the occurrence of partial chiasma interference in this species. Heterogeneity of y is observed in one of 42 cases in which markers were typed in more than one family, suggesting variation in gene–centromere recombination among families. Centromere location was mostly in accordance with the C. farreri karyotype, but differences in marker order between linkage and centromere maps occurred. Overall, this study makes the genetic linkage map a more complete and informative tool for genomic studies and it will also facilitate future research of the structure and function of the scallop centromeres.

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Acknowledgments

This study was supported by research grants from the National Natural Science Foundation of China (31072207), 973 Program (2010CB126406), and National High Technology Research and Development Program (2010AA10A110).

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  1. Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266003, China

    Hongtao Nie, Qi Li, Xuelin Zhao & Lingfeng Kong

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  1. Hongtao Nie
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  2. Qi Li
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Correspondence to Qi Li.

Appendix

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Table 1 Genotypic ratios of 90 microsatellite loci in gynogenetic and control families of C. farreri
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Nie, H., Li, Q., Zhao, X. et al. Genetic Positioning of Centromeres through Half-Tetrad Analysis in Gynogenetic Diploid Families of the Zhikong Scallop (Chlamys farreri). Mar Biotechnol 15, 1–15 (2013). https://doi.org/10.1007/s10126-012-9454-3

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