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Both Decrease in ACL1 Gene Expression and Increase in ICL1 Gene Expression in Marine-Derived Yeast Yarrowia lipolytica Expressing INU1 Gene Enhance Citric Acid Production from Inulin

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Abstract

In this study, some of the ATP-citrate lyase genes (ACL1) were deleted and the copy number of the iso-citrate lyase gene (ICL1) was increased in the marine-derived yeast Yarrowia lipolytica SWJ-1b displaying the recombinant inulinase. It was found that lipid content and iso-citric acid in the transformant 30 obtained were greatly reduced and citric acid production was greatly enhanced. It was also found that the ACL1 gene expression and ATP-citrate lyase activity in the transformant 30 were declined and the ICL1 gene expression and iso-citrate lyase activity were promoted. During the 2-l fermentation, 84.0 g/l of citric acid and 1.8 g/l of iso-citric acid in the fermented medium were attained from 10.0 % of inulin by the transformant 30 within 214 h. The results showed that only 0.36 % of the residual reducing sugar and 1.0 % of the residual total sugar were left in the fermented medium, suggesting that 89.6 % of the total sugar was used for citric acid production and cell growth by the transformant 30.

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Acknowledgments

This research was supported by Grant 31070029 from the National Natural Science Foundation of China and Hi-Tech Research and Development Program of China (863) (grant no. 2012AA021205).

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Authors and Affiliations

  1. Unesco Chinese Center of Marine Biotechnology, Institute of Evolution and Marine Biodiversity, Ocean University of China, Yushan Road, No. 5, Qingdao, China

    Xiao-Yan Liu, Zhe Chi, Guang-Lei Liu, Catherine Madzak & Zhen-Ming Chi

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  1. Xiao-Yan Liu
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  2. Zhe Chi
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  3. Guang-Lei Liu
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  4. Catherine Madzak
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  5. Zhen-Ming Chi
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Correspondence to Zhen-Ming Chi.

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Liu, XY., Chi, Z., Liu, GL. et al. Both Decrease in ACL1 Gene Expression and Increase in ICL1 Gene Expression in Marine-Derived Yeast Yarrowia lipolytica Expressing INU1 Gene Enhance Citric Acid Production from Inulin. Mar Biotechnol 15, 26–36 (2013). https://doi.org/10.1007/s10126-012-9452-5

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