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Cold-adapted Features of Arginine Kinase from the Deep-sea Clam Calyptogena kaikoi

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Abstract

The heterodont clam Calyptogena kaikoi, which inhabits depths exceeding 3,500 m where low ambient temperatures prevail, has an unusual two-domain arginine kinase (AK) with molecular mass of 80 kDa, twice that of typical AKs. The purpose of this work is to investigate the nature of the adaptations of this AK for functioning at low temperatures. Recombinant C. kaikoi AK constructs were expressed, and their two-substrate kinetic constants (k cat, K a, and K ia) were determined at 10°C and 25°C, respectively. When measured at 25°C, the K ia values were tenfold larger than those for corresponding K a values, while at 10°C, the K ia values decreased remarkably, but the K a values were almost unchanged. The Calyptogena two-domain enzyme has threefold higher catalytic efficiency, calculated by k cat/(K ARGa ·K ATPia ), at 10°C, than that at 25°C, reflecting adaptation for function at reduced ambient temperatures. The activation energy (E a) and thermodynamic parameters were determined for Calyptogena two-domain enzyme and compared with those of two-domain enzymes from mesophilic Corbicula and Anthopleura. The value for E a of Calyptogena enzyme were about half of those for mesophilic enzymes, and a larger decrease in entropy was observed in Calyptogena AK reaction. Although large decrease in entropy increases the ΔG o‡ value and consequently lowers the k cat value, this is compensated with its lower E a value thereby minimizing the reduction in its k cat value. These thermodynamic properties, together with the kinetic ones, are also present in the separated domain 2 of the Calyptogena two-domain enzyme.

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Abbreviations

AK:

Arginine kinase

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Acknowledgments

We thank W. Ross Ellington, Florida State University, for critically reading the manuscript. This work was supported by a Grant-in-Aid for Scientific Research in Japan to TS (17570062 and 20570072).

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  1. Laboratory of Biochemistry, Faculty of Science, Kochi University, Kochi, 780-8520, Japan

    Tomohiko Suzuki, Kentaro Yamamoto, Hiroshi Tada & Kouji Uda

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  1. Tomohiko Suzuki
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  2. Kentaro Yamamoto
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  3. Hiroshi Tada
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  4. Kouji Uda
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Correspondence to Tomohiko Suzuki.

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Suzuki, T., Yamamoto, K., Tada, H. et al. Cold-adapted Features of Arginine Kinase from the Deep-sea Clam Calyptogena kaikoi . Mar Biotechnol 14, 294–303 (2012). https://doi.org/10.1007/s10126-011-9411-6

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  • DOI: https://doi.org/10.1007/s10126-011-9411-6

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