Abstract
As the β-1, 3-glucanase produced by the marine-derived Williopsis saturnus WC91-2 could inhibit the activity of the killer toxin produced by the same yeast, the WsEXG1 gene encoding exo-β-1, 3-glucanase in W. saturnus WC91-2 was disrupted. The disruptant WC91-2-2 only produced a trace amount of β-1, 3-glucanase but had much higher activity of killer toxin than W. saturnus WC91-2. After the disruption of the WsEXG1 gene, the expression of the gene was significantly decreased from 100% in the cells of W. saturnus WC91-2 to 27% in the cells of the disruptant WC91-2-2 while the expression of the killer toxin gene in W. saturnus WC91-2 and the disruptant WC91-2-2 was almost the same. During 2-l fermentation, the disruptant WC91-2-2 could produce the highest amount of killer toxin (the size of the inhibition zone was 22 ± 0.7 mm) within 36 h when the cell growth reached the middle of the log phase.
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This work was supported by grant 30771645 from National Natural Science Foundation of China.
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Xu, JL., Zhang, X., Sun, HY. et al. Disruption of the Gene Encoding β-1, 3-Glucanase in Marine-Derived Williopsis saturnus WC91-2 Enhances its Killer Toxin Activity. Mar Biotechnol 14, 261–269 (2012). https://doi.org/10.1007/s10126-011-9409-0
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DOI: https://doi.org/10.1007/s10126-011-9409-0