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Cloning and Characterization of a Novel Oligoalginate Lyase from a Newly Isolated Bacterium Sphingomonas sp. MJ-3

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Abstract

A bacterium possessing alginate-degrading activity was isolated from marine brown seaweed soup liquefied by salted and fermented anchovy. The isolated strain was designated as Sphingomonas sp. MJ-3 based on the analyses of 16S ribosomal DNA sequences, 16S-23S internal transcribed spacer region sequences, biochemical characteristics, and cellular fatty acid composition. A novel alginate lyase gene was cloned from genomic DNA library and then expressed in Escherichia coli. When the deduced amino acid sequence was compared with the sequences on the databases, interestingly, the cloned gene product was predicted to consist of AlgL (alginate lyase L)-like and heparinase-like protein domain. The MJ-3 alginate lyase gene shared below 27.0% sequence identity with exolytic alginate lyase of Sphingomonas sp. A1. The optimal pH and temperature for the recombinant MJ-3 alginate lyase were 6.5 and 50°C, respectively. The final degradation products of alginate oligosaccharides were analyzed by electrospray ionization mass spectrometry and proved to be alginate monosaccharides. Based on the results, the recombinant alginate lyase from Sphingomonas sp. MJ-3 is regarded as an oligoalginate lyase that can degrade oligoalginate and alginate into alginate monosaccharides.

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Acknowledgments

This work was supported by a grant from (Development of Marine-Bioenergy) program funded by Ministry of Land, Transport and Maritime Affairs of Korean Government. This work was also financially supported by the Ministry of Knowledge Economy (MKE) and Korea Institute for Advancement in Technology (KIAT) through the Workforce Development Program in Strategic Technology.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Kyungsung University, Busan, 608-736, South Korea

    Hwan Hee Park, Natania Kam & Hee Sook Kim

  2. Department of Chemical Engineering, Kyung Hee University, Yongin-si, Gyeonggi-do, 446-701, South Korea

    Eun Yeol Lee

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  1. Hwan Hee Park
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  2. Natania Kam
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  3. Eun Yeol Lee
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  4. Hee Sook Kim
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Correspondence to Eun Yeol Lee or Hee Sook Kim.

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Park, H.H., Kam, N., Lee, E.Y. et al. Cloning and Characterization of a Novel Oligoalginate Lyase from a Newly Isolated Bacterium Sphingomonas sp. MJ-3. Mar Biotechnol 14, 189–202 (2012). https://doi.org/10.1007/s10126-011-9402-7

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  • DOI: https://doi.org/10.1007/s10126-011-9402-7

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