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Development and Linkage Analysis of 104 New Microsatellite Markers for Bay Scallop (Argopecten irradians)

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Abstract

For genetic analysis and linkage mapping of bay scallop (Argopecten irradians), a set of 120 novel simple sequence repeat markers were developed from microsatellite-enriched libraries and expressed sequence tags. An inter-subspecies hybrid bay scallop family (CC5) of 46 progeny was analyzed as the reference population to confirm polymorphism and test the segregation patterns of these loci. A total of 104 microsatellite markers were polymorphic in the reference family, among which 36 in female, 28 in male, and 40 in both parents, respectively. Linkage analysis allowed mapping these markers to 15 linkage groups, which is close to the haploid chromosome number of bay scallop (n = 16). Analysis of the 40 markers segregating in both parents showed a higher recombination rate in the female parent, with the average of female-to-male recombination ratio of 1.09:1 between linked pairs of markers. When null alleles were considered, there were 17 loci showing segregation distortion at the 5% significance level using the chi-square test. The microsatellite markers developed in this study provide a useful resource for future linkage mapping and quantitative loci analysis in A. irradians.

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Acknowledgments

We thank all the staff of Laboratory of Marine Genetics and Breeding, Ocean University of China for technical help with constructing microsatellite enrichment libraries. We thank Zhenmin Bao and Jingjie Hu for their support during the course of this work. We thank Huaiping Zhen for his kind supply of bay scallops and Xuedi Du and Rui Song for their assistance in part of the microsatellite genotyping. This research was supported by grants from National High-Technology R&D Plan (2006AA10A407 to X.L.) and National Science Foundation of China (30371117 to X.L.).

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Authors and Affiliations

  1. Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Hongjun Li, Xiao Liu & Guofan Zhang

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  1. Hongjun Li
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  2. Xiao Liu
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  3. Guofan Zhang
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Correspondence to Xiao Liu.

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Li, H., Liu, X. & Zhang, G. Development and Linkage Analysis of 104 New Microsatellite Markers for Bay Scallop (Argopecten irradians). Mar Biotechnol 14, 1–9 (2012). https://doi.org/10.1007/s10126-011-9383-6

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  • DOI: https://doi.org/10.1007/s10126-011-9383-6

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