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Structural Characterization of Extracellular Polysaccharides Produced by the Marine Fungus Epicoccum nigrum JJY-40 and Their Antioxidant Activities

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Abstract

Two extracellular polysaccharides, ENP1 and ENP2, were isolated from the fermentation liquid of the marine fungus Epicoccum nigrum JJY-40 by anion-exchange chromatography and gel-filtration chromatography, and their structures were investigated using chemical and spectroscopic methods including methylation analysis and NMR spectroscopy. The results demonstrated that ENP1 was composed of mannose, glucose, and galactose in the molar ratio of 5.0:2.1:1.0, and the main chain of the polysaccharide consisted of (1 → 2)-linked mannose, (1 → 3)-linked mannose, terminal mannose, (1 → 6)-linked glucose, (1 → 4)-linked glucose, and (1 → 4)-linked galactose. ENP2 was composed of mannose, galactose, glucose, and glucuronic acid in a molar ratio of 12.4:11.2:8.3:1.0, and its glycosidic linkage patterns included terminal mannose, (1 → 6)-linked glucose, (1 → 4)-linked galactose, and (1 → 3)-linked mannose. The two polysaccharides had a partially branched structure with branch point located at C-3 position of (1 → 6)-linked glucose residue. The molecular weights of ENP1 and ENP2 were 19.2 kDa and 32.7 kDa, respectively. Antioxidant properties of the two polysaccharides were evaluated with hydroxyl, superoxide, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities and lipid peroxidation inhibition in vitro, and results showed that ENP2 and ENP1 had good antioxidant activities, especially ENP2. ENP2 could be effective as a potential antioxidant.

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Acknowledgments

This work was supported in part by the Science and Technology Development Program of Shandong Province, China (2010GHY10509, 2006GG2205014), the National Science and Technology Infrastructure Program of China (2008BAD94B04), the National Natural Science Foundation of China (41076086), and the Doctoral Program of Higher Education of China (20090132110015). The authors gratefully thank Professor Gu Qianqun, School of Medicine and Pharmacy, Ocean University of China, for providing the fermentation broth of the marine fungus Epicoccum nigrum JJY-40.

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Authors and Affiliations

  1. Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, Qingdao, 266003, China

    Hai-Hong Sun, Wen-Jun Mao, Jie-Ying Jiao, Hong-Yan Li, Yin Chen, Xiao-Hui Qi, Yan-Li Chen, Jian Xu, Chun-Qi Zhao, Yu-Jiao Hou & Yu-Pin Yang

  2. College of Food Science and Technology, Ocean University of China, Qingdao, 266003, China

    Jia-Chao Xu

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  1. Hai-Hong Sun
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Correspondence to Wen-Jun Mao.

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Sun, HH., Mao, WJ., Jiao, JY. et al. Structural Characterization of Extracellular Polysaccharides Produced by the Marine Fungus Epicoccum nigrum JJY-40 and Their Antioxidant Activities. Mar Biotechnol 13, 1048–1055 (2011). https://doi.org/10.1007/s10126-011-9368-5

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  • DOI: https://doi.org/10.1007/s10126-011-9368-5

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