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Induced Gynogenesis in Grass Carp (Ctenopharyngodon idellus) Using Irradiated Sperm of Allotetraploid Hybrids

Marine Biotechnology volume 13, pages 1017–1026 (2011)Cite this article

Abstract

Grass carp (Ctenopharyngodon idellus) eggs were activated by UV-irradiated diploid sperm of allotetraploid hybrids derived from red crucian carp (♀) × common carp (♂) and then duplicated by cold shock in 4–6°C water for 10–12 min. Different cold shock initiation times resulted in two types of diploid gynogenetic grass carp: meiotic gynogenetic (meiG) and mitotic gynogenetic (mitG). Over a 5-year period, a total of 17,170 meiG and 1,080 mitG fry were produced and 6,862 meiG and 372 mitG grass carp survived. The gynogenetic fish were confirmed by morphological characteristics, chromosome examination, and microsatellite DNA analysis. The morphological traits of the gynogenetic grass carp were similar to those of wild diploid grass carp. Normal gynogenetic fish were identified as diploid with 48 chromosomes by chromosomal metaphases examination, while nonviable abnormal embryos were detected as haploid with 24 chromosomes. Microsatellite DNA analysis indicated that after one generation of gynogenesis, the genetic purity of meiG and mitG grass carp was significantly increased over that of wild grass carp. In addition, both meiG and mitG grass carp groups were 100% female, and 88% of these showed normal ovary development. Thus, the sex determination mechanism in female grass carp was homogamety. The ability to establish pure all-female groups of meiG and mitG grass carp should be a valuable contribution to both fish genetics and grass carp breeding.

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Acknowledgments

This research was supported by grants from the National Science Fund for Distinguished Young Scholars (no. 30725028), the National Natural Science Foundation of China (no. 30930071), and the State Key Basic Research Project of China (abbreviate: 973 project, grant no. 2007CB109206).

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  1. Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, 410081, People’s Republic of China

    Hong Zhang, ShaoJun Liu, Chun Zhang, Min Tao, LiangYue Peng, CuiPing You, Jun Xiao, Yi Zhou, GongJian Zhou, KaiKun Luo & Yun Liu

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  1. Hong Zhang
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  2. ShaoJun Liu
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  3. Chun Zhang
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  4. Min Tao
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  7. Jun Xiao
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Correspondence to ShaoJun Liu.

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Zhang, H., Liu, S., Zhang, C. et al. Induced Gynogenesis in Grass Carp (Ctenopharyngodon idellus) Using Irradiated Sperm of Allotetraploid Hybrids. Mar Biotechnol 13, 1017–1026 (2011). https://doi.org/10.1007/s10126-011-9365-8

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Keywords

  • Grass carp
  • Gynogenesis
  • Microsatellite
  • Sex determination