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Genetic Linkage Mapping and Analysis of Muscle Fiber-Related QTLs in Common Carp (Cyprinus carpio L.)

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Abstract

A genetic linkage map of common carp (Cyprinus carpio L.) was constructed using Type I and Type II microsatellite markers and a pseudo-testcross mapping strategy. The microsatellite markers were isolated from microsatellite-enriched genomic libraries and tested for their segregation in a full-sib mapping panel containing 92 individuals. A total of 161 microsatellite loci were mapped into 54 linkage groups. The total lengths of the female, male and consensus maps were 2,000, 946, and 1,852 cM, with an average marker spacing of approximately 13, 7, and 11 cM, respectively. Muscle fiber-related traits, including muscle fiber cross-section area and muscle fiber density, were mapped to the genetic map. Three QTLs for muscle fiber cross-section area and two QTLs for muscle fiber density were identified when considering both significant and suggestive QTL effects. The QTLs with largest effects for muscle fiber cross-section area and muscle fiber density were 21.9% and 18.9%, and they were located in LG3, respectively.

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Acknowledgments

Thanks are given to Jian Wu (Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences) for her guide in data analysis. This study was supported by The National Basic Research Program of China (2004CB117404), National Department Public Benefit Research Foundation (200903045) and Research Foundation of CAFS (2009C001 and 2010C016).

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Authors and Affiliations

  1. The Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, Beijing, 100141, China

    Yan Zhang, Peng Xu, Hengde Li, Shu Wang & Xiaowen Sun

  2. Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, 150070, China

    Yan Zhang, Cuiyun Lu, Youyi Kuang, Xiaofeng Zhang, Dingchen Cao, Chao Li, Yumei Chang, Ning Hou & Xiaowen Sun

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  1. Yan Zhang
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  2. Peng Xu
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  3. Cuiyun Lu
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Correspondence to Xiaowen Sun.

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Table 3 Sequence and amplified result of microsatellite loci
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Zhang, Y., Xu, P., Lu, C. et al. Genetic Linkage Mapping and Analysis of Muscle Fiber-Related QTLs in Common Carp (Cyprinus carpio L.). Mar Biotechnol 13, 376–392 (2011). https://doi.org/10.1007/s10126-010-9307-x

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