Abstract
Marine coccolithophorids (Haptophyceae) produce calcified scales “coccoliths” which are composed of CaCO3 and coccolith polysaccharides (CP) in the coccolith vesicles. CP was previously reported to be composed of uronic acids and sulfated residues, etc. attached to the polymannose main chain. Although anionic polymers are generally known to play key roles in biomineralization process, there is no experimental data how CP contributes to calcite crystal formation in the coccolithophorids. CP used was isolated from the most abundant coccolithophorid, Emiliania huxleyi. CaCO3 crystallization experiment was performed on agar template layered onto a plastic plate that was dipped in the CaCO3 crystallization solution. The typical rhombohedral calcite crystals were formed in the absence of CP. CaCO3 crystals formed on the naked plastic plate were obviously changed to stick-like shapes when CP was present in the solution. EBSD analysis proved that the crystal is calcite of which c-axis was elongated. CP in the solution stimulated the formation of tabular crystals with flat edge in the agarose gel. SEM and FIB-TEM observations showed that the calcite crystals were formed in the gel. The formation of crystals without flat edge was stimulated when CP was preliminarily added in the gel. These observations suggest that CP has two functions: namely, one is to elongate the calcite crystal along c-axis and another is to induce tabular calcite crystal formation in the agarose gel. Thus, CP may function for the formation of highly elaborate species-specific structures of coccoliths in coccolithophorids.
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Acknowledgment
The authors are grateful to Dr. Iwane Suzuki for his continuous help and valuable discussion during this study. This study was supported in part by the Global Environment Research Fund from the Japanese Ministry of Environment (FY2008-10; F-083) to YS.
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Kayano, K., Saruwatari, K., Kogure, T. et al. Effect of Coccolith Polysaccharides Isolated from the Coccolithophorid, Emiliania huxleyi, on Calcite Crystal Formation in In Vitro CaCO3 Crystallization. Mar Biotechnol 13, 83–92 (2011). https://doi.org/10.1007/s10126-010-9272-4
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DOI: https://doi.org/10.1007/s10126-010-9272-4