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Ectoine Production by Halomonas boliviensis: Optimization Using Response Surface Methodology

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Abstract

Two cultivation steps were used for production of biomass and ectoine by Halomonas boliviensis, respectively. The optimization of some nutrient parameters in each step was investigated by using response surface methodology. Twenty and 12 experiments were performed to attain optimal conditions for biomass and ectoine production, respectively. The model predicted a maximum biomass concentration of 3.34 g/L on optimization of NH4Cl, K2HPO4, and MgSO4•7H2O concentrations during the first cultivation, while a maximum ectoine concentration of 1.27 g/L was predicted on optimizing NaCl and monosodium glutamate concentrations in the second cultivation. The experimental values obtained (3.36 g biomass/L and 1.25 g ectoine/L) were in good agreement with the predicted values. The optimized conditions were also used for two-step 1.5-L fed-batch fermentations. In the first step, biomass concentration of 28.7 g/L was obtained while in the second step biomass concentration increased to 63 g/L. Ectoine concentration of 9.2 g/L was obtained, and the overall ectoine productivity was 6.3 g/L/day, being among the highest reported so far.

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Acknowledgment

The authors would like to thank the Swedish International Development Cooperation Agency (Sida) for supporting this work.

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Authors and Affiliations

  1. Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, 221 00, Lund, Sweden

    Doan Van-Thuoc, Héctor Guzmán & Rajni Hatti-Kaul

  2. Department of Micro-biotechnology, Faculty of Biology, Hanoi National University of Education, 136 Xuan Thuy, Cau Giay, Hanoi, Vietnam

    Doan Van-Thuoc & Mai Thi-Hang

  3. Centro de Biotecnología, Facultad de Ciencias y Tecnología, Universidad Mayor de San Simón, Cochabamba, Bolivia

    Héctor Guzmán

Authors
  1. Doan Van-Thuoc
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  2. Héctor Guzmán
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  3. Mai Thi-Hang
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  4. Rajni Hatti-Kaul
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Corresponding author

Correspondence to Doan Van-Thuoc.

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Table 1

Regression coefficients for biomass production by H. boliviensis (DOC 36 kb)

Table 2

ANOVA for biomass production by H. boliviensis (DOC 35 kb)

Table 3

Regression coefficients for ectoine production by H. boliviensis (DOC 31 kb)

Table 4

ANOVA for ectoine production by H. boliviensis (DOC 33 kb)

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Van-Thuoc, D., Guzmán, H., Thi-Hang, M. et al. Ectoine Production by Halomonas boliviensis: Optimization Using Response Surface Methodology. Mar Biotechnol 12, 586–593 (2010). https://doi.org/10.1007/s10126-009-9246-6

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