Preliminary characterization of a biosurfactant-producing Azotobacter chroococcum isolated from marine environment showed maximum biomass and biosurfactant production at 120 and 132 h, respectively, at pH 8.0, 38°C, and 30‰ salinity utilizing a 2% carbon substrate. It grew and produced biosurfactant on crude oil, waste motor lubricant oil, and peanut oil cake. Peanut oil cake gave the highest biosurfactant production (4.6 mg/mL) under fermentation conditions. The biosurfactant product emulsified waste motor lubricant oil, crude oil, diesel, kerosene, naphthalene, anthracene, and xylene. Preliminary characterization of the biosurfactant using biochemical, Fourier transform infrared spectroscopy, and mass spectral analysis indicated that the biosurfactant was a lipopeptide with percentage lipid and protein proportion of 31.3:68.7.
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We thank the authorities of Annamalai University for providing the facilities and DOD and CSIR, Government of India for financial support.
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Thavasi, R., Subramanyam Nambaru, V.R.M., Jayalakshmi, S. et al. Biosurfactant Production by Azotobacter chroococcum Isolated from the Marine Environment. Mar Biotechnol 11, 551 (2009). https://doi.org/10.1007/s10126-008-9162-1
- Crude oil
- Waste motor lubricant oil
- Peanut oil cake