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Simplex PCR Assay for Positive Identification of Genetic Sex in the Japanese Medaka, Oryzias latipes

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Abstract

The medaka, Oryzias latipes, is a very popular model in biomedical research, particularly for elucidating sex differentiation and determination mechanisms and effects of endocrine disruptors among others. These studies require a sensitive, accurate, rapid, and reliable technique for genetic sexing of eggs, larvae, and adults. In this study, we report a simplex polymerase chain reaction approach that uses a single pair of primers for simultaneous amplification of sex-specific amplicons. Males and females yield a single diagnostic band of 933 and 1,906 bp, respectively, in three different strains of medaka tested, permitting gender identification accurately of both immature and adult fish. This technique will be useful in both ecological and biomedical researches that employ medaka and rely on genetic sexing.

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Acknowledgments

The authors wish to thank Prof. A. Shima and Dr H. Mitani of Tokyo University for the gift of inbred strains of medaka and M. Canning for animal husbandry. We also thank R.Thresher, R. Gurney, and G. Abell for comments on an earlier draft of this manuscript. This work was funded by CSIRO and the Invasive Animal Cooperative Research Centre.

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Authors and Affiliations

  1. CSIRO, Marine and Atmospheric Research Laboratories, Hobart, Tasmania, 7001, Australia

    Jawahar G. Patil & Susan J. Hinze

  2. Invasive Animal CRC, Canberra, ACT, Australia

    Jawahar G. Patil & Susan J. Hinze

Authors
  1. Jawahar G. Patil
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  2. Susan J. Hinze
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Correspondence to Jawahar G. Patil.

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Patil, J.G., Hinze, S.J. Simplex PCR Assay for Positive Identification of Genetic Sex in the Japanese Medaka, Oryzias latipes . Mar Biotechnol 10, 641–644 (2008). https://doi.org/10.1007/s10126-008-9106-9

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  • DOI: https://doi.org/10.1007/s10126-008-9106-9

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