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Characterization of Cupric Glutamate Extinguishing Mechanism of Alexandrium sp. LC3 with Two-dimensional Electrophoresis and MALDI-TOF MS

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Abstract

Mechanisms by which cupric glutamate, a novel algicide, extinguishes Alexandrium sp. LC3 are shown in this study. We show that cupric glutamate not only stimulated the production of malonaldehyde (MDA) and dramatically promoted cell plasma membrane permeability (p < 0.01) but also remarkably reduced sulfhydryl (SH) group content (p < 0.01). Analysis of protein expression profiles by two-dimensional electrophoresis (2-DE) indicated that only 47 protein spots were detected in both control and cupric glutamate treated cells. Three reliable spots were identified by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) as ribulose-bisphosphate carboxylase large subunit precursor, RNA polymerase beta chain, and hypothetical protein, which can be well correlated with cupric glutamate stress. Based on above results, we hypothesize that the extinguishing mechanisms include (1) the cell membrane being damaged by cupric glutamate; (2) cupric glutamate probably induced denaturation and disintegration of intracellular protein, which led to inhibition of cell growth.

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Acknowledgment

This work was supported by grant from National Natural Science Foundation of China (40206022).

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Author notes

  1. Jinlai Miao

    Present address: Key Laboratory of Marine Biological Active Substances, SOA., 6 Xian Xia-ling Road, Hi-Tech Park, Qingdao, 266061, China

  2. Hao Li

    Present address: Beijing Proteome Research Center, 33 Life Science Park Road, Beijing, 102206, China

Authors and Affiliations

  1. Key Laboratory of Marine Biological Active Substances, SOA., 6 Xian Xia-ling Road, Hi-Tech Park, Qingdao, 266061, China

    Hao Li & Guangyou Li

  2. Marine Life Sciences College, Ocean University of China, Qingdao, 266003, China

    Hao Li

  3. China National Research Institute of Food and Fermentation Industries, Beijing, 100027, China

    Fengxia Cui

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  1. Hao Li
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Correspondence to Jinlai Miao.

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Li, H., Miao, J., Cui, F. et al. Characterization of Cupric Glutamate Extinguishing Mechanism of Alexandrium sp. LC3 with Two-dimensional Electrophoresis and MALDI-TOF MS. Mar Biotechnol 10, 527–537 (2008). https://doi.org/10.1007/s10126-008-9091-z

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