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Characterization of Calcium Deposition and Shell Matrix Protein Secretion in Primary Mantle Tissue Culture from the Marine Pearl Oyster Pinctada fucata

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Abstract

In this study, we established and characterized a long-term primary mantle tissue culture from the marine pearl oyster Pinctada fucata for in vitro investigation of nacre biomineralization. In this culture system, the viability of mantle tissue cells lasted up to 2 months. The tissue cells were demonstrated to express nacre matrix proteins by RT-PCR, and a soluble shell matrix protein, nacrein, was detected in the culture medium by Western blot analysis. On the other hand, 15 days after initiating culture, a large amount of calcium deposits with major elements, including calcium, carbon, and oxygen, were generated in the mantle explants and cell outgrowth area. The quantity and size of calcium deposits increased with the prolonged cultivation, and their location and nanogranular structure suggested their biogenic origin. These calcium deposits specifically appeared in mantle tissue cultures, but not in heart tissue cultures. Taken together, these results demonstrate that the mantle tissue culture functions similarly to mantle cells in vivo. This study provides a reliable approach for the further investigation on nacre biomineralization at the cellular level.

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Acknowledgments

This work was financially supported by the National High Technology Research and Development Program of China (2003AA603430, 2006AA09Z413) and the Natural Science Foundation of China (30221003, 30530600). The authors thank Professor Dr. Lina Ma (Jilin University) and Dr. Mark Bartlam (School of Medicine, Tsinghua University) for critical discussion.

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Authors and Affiliations

  1. Institute of Marine Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, 100084, China

    Ningping Gong, Zhuojun Ma, Qing Li, Qi Li, Zhenguang Yan, Liping Xie & Rongqing Zhang

  2. Protein Science Laboratory of the Ministry of Education, Tsinghua University, Beijing, 100084, China

    Liping Xie & Rongqing Zhang

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  1. Ningping Gong
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  2. Zhuojun Ma
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  3. Qing Li
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  4. Qi Li
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  5. Zhenguang Yan
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  6. Liping Xie
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  7. Rongqing Zhang
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Correspondence to Liping Xie or Rongqing Zhang.

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Gong, N., Ma, Z., Li, Q. et al. Characterization of Calcium Deposition and Shell Matrix Protein Secretion in Primary Mantle Tissue Culture from the Marine Pearl Oyster Pinctada fucata . Mar Biotechnol 10, 457–465 (2008). https://doi.org/10.1007/s10126-008-9081-1

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  • DOI: https://doi.org/10.1007/s10126-008-9081-1

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