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The Encysted Dormant Embryo Proteome of Artemia sinica

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Abstract

The possibility of the brine shrimp Artemia to produce dormant embryo (cysts) in diapause is a key feature in its life history. In the present study, we obtained a proteomic reference map for the diapause embryo of Artemia sinica using two-dimensional gel electrophoresis with a pH range of 4–7 and a molecular weight range of 10–100 kDa. Approximately 233 proteins were detected, and 60 of them were analyzed by capillary liquid chromatography tandem mass spectrometry (LC–MS/MS). Of these, 39 spots representing 33 unique proteins were identified, which are categorized into functional groups, including cell defense, cell structure, metabolism, protein synthesis, proteolysis, and other processes. This reference map will contribute toward understanding the state of the diapause embryo and lay the basis and serve as a useful tool for further profound studies in the proteomics of Artemia at different developmental stages and physiological conditions.

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Acknowledgments

This research was supported by the program of National Natural Science Foundation of China (20060109Z4016) and Natural Science Foundation of Shandong Province for the excellent young researcher (2006BSA02004).

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Authors and Affiliations

  1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, People’s Republic of China

    Qian Zhou, Changgong Wu, Bo Dong & Jianhai Xiang

  2. Graduate School, Chinese Academy of Sciences, Beijing, 100039, People’s Republic of China

    Qian Zhou

  3. College of Life Sciences, Nankai University, Tianjin, 300071, People’s Republic of China

    Fengqi Liu

Authors
  1. Qian Zhou
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  2. Changgong Wu
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  3. Bo Dong
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  4. Fengqi Liu
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  5. Jianhai Xiang
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Correspondence to Jianhai Xiang.

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Zhou, Q., Wu, C., Dong, B. et al. The Encysted Dormant Embryo Proteome of Artemia sinica . Mar Biotechnol 10, 438–446 (2008). https://doi.org/10.1007/s10126-007-9079-0

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  • DOI: https://doi.org/10.1007/s10126-007-9079-0

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