Abstract
A novel alternative for wastewater effluent bioremediation was developed using constructed microbial mats on low-density polyester. This biotechnology showed high removal efficiencies for nitrogen and phosphorous in a short retention time (48 h): 94% for orthophosphate (7.78 g \( PO_{4} ^{{3 - }} - P \) m3 d−1), 79% for ammonium (11.30 g \( NH_{4} ^{ + } - N \) m−3 d−1), 78% for nitrite (7.46 g \( NO_{2} ^{ - } - N \) m−3 d−1), and 83% for nitrate (8.55 g \( NO_{3} ^{ - } - N \) m−3 d−1). The microbial mats were dominated by Cyanobacteria genera such as Chroococcus sp., Lyngbya sp., and bacteria of the subclass Proteobacteria representative of the Eubacteria Domain. Nitzschia sp. was the dominant Eukaryote Domain. Various N and P substrates in the wastewater permit the growth of self-forming and self-sustaining bacterial, microalgal, and cyanobacterial communities on a polyester support. The result is the continuous, self-sufficient growth of microbial mats. This is an innovative, economical, and environmentally safe alternative for the treatment of wastewater effluents in coastal marine environments.
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References
APHA (American Public Health Association, American Water Works Association, Water Pollutions Control Federations) (1995) Standard Methods for the Examination of Water and Wastewater, 19 th ed (Washington, DC: APHA)
Ausubel, FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1992) Short Protocols in Molecular Biology (New York: Greene Publishing Associates and John Wiley & Sons)
Bender J, Phillips P (1995) Biological remediation of mixed wastes by microbial mats. Fed. Facilities 217/Environ J Autumn, 77–85
Bender J, Phillips P (2004) Microbial mats for multiple applications in aquaculture and bioremediation. Bioresource Technol 94, 229–238
Bender J, Vatcharapijarn Y, Russell A (1989a) Fish feeds from grass clippings. Aquacult Eng 8, 407–419
Craggs RJ, McAuley PJ, Smith VJ (1997) Wastewater nutrient removal by marine microalgae grown on a corrugated raceway. Water Res 31, 1701–1707
Daims H, Nielsen JL, Nielsen PH, Schleifer KH, Wagner M (2001) In situ characterization of Nitrospira-like nitrite-oxidizing bacteria active in wastewater treatment plants. Appl Environ Microbiol 67, 5273–5284
Ebeling JM, Rishel KL, Sibrell PL (2005) Screening and evaluation of polymers as flocculation aids. Aquacult Eng 33, 235–249
Franco-Rivera A, Paniagua-Michel J, Zamora-Castro J (2007) Characterization and performance of constructed nitrifying biofilms during nitrogen bioremediation of a wastewater effluent. J Ind Microbiol Biotechnol 34, 279–287.
Gómez-Cerezo R, Suárez ML, Vidal-Abarca MR (2001) The performance of a multistage system of constructed wetlands for urban wastewater treatment in semiarid region of SE Spain. Ecol Eng 16, 501–517
Guillard R, Ryther JH (1962) Studies of marine planktonic diatoms. I. Cyclotellanana (Husted) and Detonula confervacea (Cleve). Can J Microbiol 8, 229–239
Hernández-Zárate G, Olmos-Soto J (2006) Identification of bacterial diversity in the oyster Crassostrea gigas by fluorescent in situ hybridization and polymerase chain reaction. J Appl Microbiol 4, 664–672
Horrigan SG, Hagström Å, Koike K, Azam F (1988) Inorganic nitrogen utilization by assemblages of marine bacteria in seawater culture. Mar Ecol Prog Ser 50, 147–150
Kloep F, Roske I, Neu T (2000) Performance and microbial structure of a nitrifying fluidized-bed reactor. Water Res 34, 311–319
Lau PS, Tam NFY, Wong YS (1998) Effect of carrageenan immobilization on the physiological activities of Chlorella vulgaris. Bioresour Technol 63, 115–121
Liu W-T, Nielsen AT, Wu J-H, Tsai C-S, Matsuo Y, Molin S (2001) In situ identification of polyphosphate- and polyhydroxyalkanoate-accumulating traits for microbial populations in a biological phosphorus removal process. Environ Microbiol 3, 110–122
Mallick N (2002) Biotechnological potential of immobilized algae for wastewater N, P and metal removal: a review. Biometals 15, 377–390
Oswald WJ (1988) Micro-algae and wastewater treatment. In: Micro-algal Biotechnology. Borowitzka MA, Borowitzka LJ eds. (Cambridge, UK: Cambridge University Press) pp 305–326
Paerl HW, Pinckney JL (1996) Microbial consortia; their roles in aquatic production and biogeochemical cycling. Microb Ecol 31, 225–247
Paniagua-Michel J, García O (2003) Ex-situ bioremediation of shrimp culture effluent using constructed microbial mats. Aquacult Eng 28, 131–139
Prescott GW (1954) How to Know the Freshwater Algae (Dubuque, IA: Wm C, Brown)
Qureshi F, Badar U, Ahmed N (2001) Biosorption of copper by bacterial biofilm on a flexible polyvinil chloride conduit. Appl Environ Microbiol 9, 4349–4352
Rippka R, Deruelles J, Waterbury JB, Herdman M, Stanier RY (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 111, 1–61
Rostron WM, Stuckey DC, Young AA (2001) Nitrification of high strength ammonia wastewater: comparative study of immobilisation media. Water Res 35, 1169–1178
Round FE, Crawford RM, Mann DG (1990) The Diatoms. Biology and Morphology of the Genera (Cambridge, UK: Cambridge University Press)
Soeder CJ, Hegewald E (1988) Scenedesmus. In: Micro-algal Biotechnology. Borowitzka MA, Borowitzka LJ eds. (Cambridge, UK: Cambridge University Press) pp 59–84
Stolz JF (2000) Structure of microbial mats and biofilms. In: Microbial Sediments. Riding RE, Awramik SM eds. (Heidelberg: Springer) pp 1–8
Syrett PJ (1981) Nitrogen metabolism of microalgae. In: Physiological Basis of Phytoplankton Ecology: Can Bull Fish Aquat Sci. Platt T ed. 210, pp 182–210
Tam NF, Wong YS (2000) Effect of immobilized microalgal bead concentrations on wastewater nutrient removal. Environ Pollut 107, 145–151
Tchobanoglous G, Burton FL, Stensel HD (2003) Wastewater Engineering: Treatment and Reuse (New York: McGraw-Hill)
Urano N, Sasaki E, Ueno R, Namba H, Shida Y (2002) Bioremediation of fish cannery wastewater with yeast isolated from drainage canal. Mar Biotech 4, 559–564
Urrutia I, Serra JL, Llama MJ (1995) Nitrate removal from water by Scenedesmus obliquus immobilized in polymeric foams. Enzyme Microb Tech 17, 200–205
Vasconcelos VM, Pereira E (2001) Cyanobacteria diversity and toxicity in a wastewater treatment plant (Portugal). Water Res 35, 1354–1357
Wagner M, Loy A (2002) Bacterial community composition and function in sewage treatment systems. Curr Opin Biotech 13, 218–227
Winter JG, Duthie HC (2000) Epilithic diatoms as indicators of stream total N and P concentration. J North Am Benthol Soc 19, 32–49
Yang P, Wah-Koon T, Yen-Peng T (2006) Design and performance study of a novel immobilized hollow fiber membrane bioreactor. Bioresource Technol 97, 39–46
Zamora-Castro JE (2004) Bioremediation System of a Coastal-Marine Wastewater Effluent by Constructed Microbial Mats (in Spanish). M. S. Thesis, Centro de Investigación Científica y de Educación Superior de Ensenada. Ensenada, B. C. México.
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The authors thank the Fondo Sectorial Secretaria Medio Ambiente y Recursos Naturales (SEMARNAT) and the National Council for Science and Technology of Mexico (CONACYT), project 0683, for financial support. We also thank Drs. Jorge Olmos and Galdy Hernandez for their kind help in the molecular characterization of bacteria, Israel Gradilla for his help in electron micrograph analysis, and Francisco Valenzuela for drawing figures. The authors also thank anonymous reviewers for their valuable comments and suggestions.
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Zamora-Castro, J., Paniagua-Michel, J. & Lezama-Cervantes, C. A Novel Approach for Bioremediation of a Coastal Marine Wastewater Effluent Based on Artificial Microbial Mats. Mar Biotechnol 10, 181–189 (2008). https://doi.org/10.1007/s10126-007-9050-0
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DOI: https://doi.org/10.1007/s10126-007-9050-0