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Induction of Attachment of the Mussel Perna perna by Natural Products from the Brown Seaweed Stypopodium zonale

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Abstract

Marine invertebrates settle, attach, and/or metamorphose in response to signals from several sources, including seaweeds. In response to the aquaculture challenge of producing constant numbers of juveniles from cultured species, natural inducers have been screened for their ability to improve those processes. However, few chemical inducers of attachment of invertebrates have been identified, and even less of these were secondary metabolites. The goal of this work was to isolate the natural products responsible for induction activity using bioassay-guided fractionation of the organic extract of the brown seaweed Stypopodium zonale and the attachment of juveniles of the common brown mussel, Perna perna, as a model. The meroditerpene epitaondiol, identified by comparison of spectral data with the literature, promoted as much as 4.7 times more mussel attachment compared to controls at the natural concentration found in this alga (0.041% of the crude extract or 0.012% of algal dry weight). This is the first report showing that a seaweed produces terpenoid compounds as cues for invertebrate attachment, and future studies evaluating this action on settlement of mussels in the field are expected to improve aquaculture technology by increasing mussel spat production.

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Acknowledgments

The authors thank CNPq and FAPERJ for financial support. B.A.P.G., V.L.T., and R.C.P. gratefully acknowledge CNPq (Brazilian National Research Council) for providing their Research Productivity fellowships. A.R.S. thanks CAPES and FAPERJ for PhD scholarships. Comments by five reviewers and N. Fusetani were invaluable in improving the manuscripts and are gratefully acknowledged.

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Authors and Affiliations

  1. Departamento de Química Orgânica, Instituto de Química, Universidade Federal do Rio de Janeiro (UFRJ)—Núcleo em Ecologia e Desenvolvimento Sócio-ambiental de Macaé, Universidade Federal do Rio de Janeiro (NUPEM-UFRJ), CEP 27901-000, Macaé, RJ, Brazil

    Angélica R. Soares

  2. Departamento de Biologia Marinha, Universidade Federal Fluminense (UFF), PO Box 100644, Niterói, RJ, CEP 24001-970, Brazil

    Bernardo A. P. da Gama, Andrea P. da Cunha, Valéria L. Teixeira & Renato C. Pereira

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  1. Angélica R. Soares
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  2. Bernardo A. P. da Gama
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  3. Andrea P. da Cunha
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  4. Valéria L. Teixeira
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  5. Renato C. Pereira
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Correspondence to Bernardo A. P. da Gama.

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Soares, A.R., da Gama, B.A.P., da Cunha, A.P. et al. Induction of Attachment of the Mussel Perna perna by Natural Products from the Brown Seaweed Stypopodium zonale . Mar Biotechnol 10, 158–165 (2008). https://doi.org/10.1007/s10126-007-9048-7

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  • DOI: https://doi.org/10.1007/s10126-007-9048-7

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