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Development of a Pluripotent ES-like Cell Line from Asian Sea Bass (Lates calcarifer)—An Oviparous Stem Cell Line Mimicking Viviparous ES Cells

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Abstract

We report a pluripotent embryonic stem cell-like cell line designated as SBES from blastula stage embryos of Asian sea bass (Lates calcarifer), which is an economically important cultivable and edible marine fish species in India. The SBES cells were cultured at 28°C in Leibovitz L-15 medium supplemented with 20% fetal bovine serum without a feeder layer. The ES-like cells were round or polygonal and grew exponentially in culture. The SBES cells exhibited an intense alkaline phosphatase activity and expression of transcription factor Oct 4. The undifferentiated state of these cells was maintained at low seeding densities and the cells formed embryoid bodies when seeded in bacteriological plates. On treatment with all-trans retinoic acid, these cells differentiated into neuron-like cells, muscle cells, and beating cardiomyocytes, indicating their pluripotency. This embryonic ES-like cell line derived from an oviparous fish blastula conserved several peculiar features of viviparous mammalian embryonic stem cell lines. The present study highlights the importance and potential of piscine ES-like cell line for stem cell research without evoking ethical issues and invasive interventions sparing mammalian embryos.

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Acknowledgment

The authors thank the management of C. Abdul Hakeem College for providing the facilities to carry out this work. The authors also thank the Director, Central Institute of Brackishwater Aquaculture, Chennai, for providing the experimental animals. Thanks are also due to Director, NCCS, Pune, for providing excellent infrastructure facilities for this study. The financial assistance provided by the Department of Biotechnology, Government of India, is gratefully acknowledged.

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Authors and Affiliations

  1. Aquaculture Biotechnology Division, Department of Zoology, C. Abdul Hakeem College, Melvisharam-632 509, Vellore Dt., India

    V. Parameswaran & A. S. Sahul Hameed

  2. Tissue Engineering & Banking Laboratory, National Centre for Cell Science, Pune, 411 007, India

    Ravi Shukla & Ramesh Bhonde

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  1. V. Parameswaran
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  2. Ravi Shukla
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  3. Ramesh Bhonde
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  4. A. S. Sahul Hameed
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Correspondence to A. S. Sahul Hameed.

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Parameswaran, V., Shukla, R., Bhonde, R. et al. Development of a Pluripotent ES-like Cell Line from Asian Sea Bass (Lates calcarifer)—An Oviparous Stem Cell Line Mimicking Viviparous ES Cells. Mar Biotechnol 9, 766–775 (2007). https://doi.org/10.1007/s10126-007-9028-y

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  • DOI: https://doi.org/10.1007/s10126-007-9028-y

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