Abstract
Growth rate of the Kuruma prawn, Marsupenaeus japonicus is an important economic trait, with larger animals commanding higher market prices. To identify gene markers associated with growth, a genetic map of a full-sib F2 intercross family of M. japonicus has previously been generated and quantitative trait loci (QTL) influencing weight, total length, and carapace length were identified. In this study, amplified fragment length polymorphism (AFLP) markers associated with the major QTL region, contributing 16% to phenotypic variation, were characterized. Flanking sequence has been obtained and allelic variants responsible for segregation patterns of these markers have been identified. The genomic sequence surrounding the AFLP band 7.21a, residing under the QTL peak, contains a gene sequence homologous to the elongation of very long chain fatty acids-like (ELOVL) protein family. A full-length mRNA (ELOVL-MJ) encoding this protein was isolated from M. japonicus, representing both the first ELOVL gene in crustacea and the first candidate gene identified via QTL studies in crustacea.
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Acknowledgments
The authors thank Dr. Kate Wilson from the Australian Institute of Marine Science for providing the wild broodstock prawn samples. The authors also thank Drs. Lisa Leeton, Belinda Norris, and Bill Barendse for their helpful comments and suggestions in development of the manuscript. This work was supported by grants from CSIRO’s Food Into Asia Program and Food Futures National Research Flagship Program.
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Lyons, R.E., Dierens, L.M., Tan, S.H. et al. Characterization of AFLP Markers Associated with Growth in the Kuruma Prawn, Marsupenaeus japonicus, and Identification of a Candidate Gene. Mar Biotechnol 9, 712–721 (2007). https://doi.org/10.1007/s10126-007-9022-4
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DOI: https://doi.org/10.1007/s10126-007-9022-4