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Isolation and Functional Characterization of a New Shrimp Ovarian Peritrophin with Antimicrobial Activity from Fenneropenaeus merguiensis

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Abstract

Shrimp ovarian peritrophin (SOP), a major protein in jelly layer (JL) and cortical rods (CRs), is proposed to play a role in the protection of spawned eggs. The full sequence of SOP cDNA from Fenneropenaeus merguiensis (Fm-SOP) shares approximately 50% identity with other SOP sequences and contains several putative chitin-binding or peritrophin-A domains. Interestingly, Fm-SOP contains a putative 61-amino acid propeptide located at the N-terminal end, downstream of a 19-amino acid signal peptide, which is unique among penaeid SOP sequences described so far. This 61-amino-acid sequence constitutes a putative chitin-binding domain with six conserved cysteines, and is cleaved at a dibasic recognition site for a furin (subtilisin-like endoprotease). Expression analyses indicated that Fm-SOP mRNA is abundant in early vitellogenic ovaries and scarce in late-vitellogenic ovaries. Conversely, Fm-SOP protein is the most abundant at the end of vitellogenesis. To investigate its biological function, a recombinant Fm-SOP was expressed to generate a glycosylated protein in Spodoptera frugiperda Sf9 cells (rSOP-Sf9) and a nonglycosylated protein (rSOP-Ec) in Escherichia coli. rSOP-Sf9 and rSOP-Ec were found to bind to chitin, similarly to the native protein extracted from F. merguiensis ovaries. Most interestingly, rSOP-Ec displayed a chitinase activity and efficiently inhibited the growth of Vibrio harveyi and Staphylococcus aureus, with minimum inhibitory concentrations of 2.4 and 15.7 μM, respectively. This first report shows that a major component of CR and JL is biologically active against known pathogens and predicts a significant role of JL in the protection of the spawned eggs against pathogens.

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Acknowledgments

We thank Dr. Bernard Romestand (University Montpellier II, France) for his assistance and highly appreciated advice on the antimicrobial tests. We thank the Trang Coastal Aquaculture Station, Trang province, Thailand, for the prawn samples, Fenneropenaeus merguiensis, and the Pathology Department, Songkhlanagarind Hospital for bacteria used in this work. This work was supported by the Royal Golden Jubilee Graduate Program from the Thailand Research Fund (TRF) for Wiriya Loongyai (4.C.PS/45/G.1), Thailand Government Research Fund 2007. We thank Dr. Brian Hodgson, Faculty of Science, Prince of Songkla University, for reading the manuscript and for valuable comments.

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Authors and Affiliations

  1. Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Hatyai, Songkla, 90112, Thailand

    Wiriya Loongyai & Wilaiwan Chotigeat

  2. Laboratoire des Symbioses Tropicales et Méditerranéenes Campus International de Baillarguet, 34398, Montpellier, Cedex 5, France

    Jean-Christophe Avarre

  3. CNRS Station de Pathologie Comparée Baculovirus et Therapie, 30380, Saint Christol Les Ales, France

    Martine Cerutti

  4. Israel Oceanographic and Limnological Research, Tel-Shikmona, P.O. Box 8030, Haifa, 31080, Israel

    Esther Lubzens

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  1. Wiriya Loongyai
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  2. Jean-Christophe Avarre
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  3. Martine Cerutti
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  4. Esther Lubzens
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  5. Wilaiwan Chotigeat
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Correspondence to Wilaiwan Chotigeat.

Additional information

The nucleotide sequence reported in this article has been submitted to the GeneBank™/EBI accession number AY775291 for Fm-SOP.

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Loongyai, W., Avarre, JC., Cerutti, M. et al. Isolation and Functional Characterization of a New Shrimp Ovarian Peritrophin with Antimicrobial Activity from Fenneropenaeus merguiensis . Mar Biotechnol 9, 624–637 (2007). https://doi.org/10.1007/s10126-007-9019-z

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