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Protective Effect of Enzymatic Extracts from Microalgae Against DNA Damage Induced by H2O2

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Abstract

The enzymatic extracts from seven species of microalgae (Pediastrum duplex, Dactylococcopsis fascicularis, Halochlorococcum porphyrae, Oltmannsiellopsis unicellularis, Achnanthes longipes, Navicula sp. and Amphora coffeaeformis) collected from three habitats (freshwater, tidal pool, and coastal benthic) at Jeju Island in Korea were investigated for their antioxidant activity. Of the extracts tested, the AMG 300 L (an exo 1, 4-α-d-glucosidase) extract of P. duplex, the Viscozyme extract of Navicula sp., and the Celluclast extract of A. longipes provided the most potential as antioxidants. Meanwhile, the Termamyl extract of P. duplex in an H2O2 scavenging assay exhibited an approximate 60% scavenging effect. In this study, we report that the DNA damage inhibitory effects of P. duplex (Termamyl extract) and D. fascicularis (Kojizyme extract) were nearly 80% and 69% respectively at a concentration of 100 μg/ml. Thus, it is suggested that the microalgae tested in this study yield promising DNA damage inhibitory properties on mouse lymphoma L 5178 cells that are treated with H2O2. Therefore, microalgae such as P. duplex may be an excellent source of naturally occurring antioxidant compounds with potent DNA damage inhibition potential.

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Acknowledgment

This research was supported by a grant (P-2004–03) from the Marine Bioprocess Research Center of the Marine Bio 21 Center, funded by the Ministry of Maritime Affairs & Fisheries, Republic of Korea.

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Author notes

  1. Abu Affan

    Present address: Korea Ocean Research and Development Institute (KORDI), Ansan P.O. Box 29, Seoul, 426–744, Republic of Korea

Authors and Affiliations

  1. Faculty of Applied Marine Science, Cheju National University, Jeju, 690–756, South Korea

    Rohan Karawita, Yasantha Athukorala & You-Jin Jeon

  2. Department of Food Science and Engineering, Cheju National University, Jeju, 690–756, South Korea

    Mahinda Senevirathne

  3. Department of Oceanography, Cheju National University, Jeju, 690–756, South Korea

    Abu Affan & Joon-Baek Lee

  4. Deparment of Veterinary Science, Cheju National University, Jeju, 690–756, South Korea

    Young-Jae Lee

  5. Department of Chemistry, Pukyong National University, Busan, 608–737, South Korea

    Se-Kwon Kim

  6. Radiological Science Research Institute, Cheju National University, Jeju, 690–756, South Korea

    You-Jin Jeon

Authors
  1. Rohan Karawita
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  2. Mahinda Senevirathne
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  3. Yasantha Athukorala
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  4. Abu Affan
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  5. Young-Jae Lee
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  7. Joon-Baek Lee
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  8. You-Jin Jeon
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Corresponding author

Correspondence to Joon-Baek Lee.

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Karawita, R., Senevirathne, M., Athukorala, Y. et al. Protective Effect of Enzymatic Extracts from Microalgae Against DNA Damage Induced by H2O2 . Mar Biotechnol 9, 479–490 (2007). https://doi.org/10.1007/s10126-007-9007-3

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  • DOI: https://doi.org/10.1007/s10126-007-9007-3

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