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Inhibitory Effect of Chitooligosaccharides on Matrix Metalloproteinase-9 in Human Fibrosarcoma Cells (HT1080)

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Abstract

Matrix metalloproteinase-9 (MMP-9) has gelatinase activity and plays an important role in cancer invasion and metastasis. Therefore, inhibition of specific types of MMPs including MMP-9 has become an attractive target for therapeutic intervention. The aim of this study was to investigate the effect of chitooligosaccharides (COS) on activity and expression of MMP-9 in HT1080 cells. The inhibitory effect of COS with different molecular masses was examined by gelatin zymography, reverse transcriptase-polymerase chain reaction (RT-PCR), gene reporter assay, and Western blot analysis. MMP-9 inhibition in the presence of COS was clearly observed in gelatin zymography. Specifically, 1- to 3-kDa COS (COS-I) exhibited the highest inhibitory effect on MMP-9 activity in HT1080 cells among tested molecular mass fractions. It was also found that COS-I was capable of inhibiting both gene and protein expression of MMP-9 (P < 0.01). These results suggest that low molecular mass COS can be considered as a potent inhibitor of MMP-9.

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Acknowledgment

The authors acknowledge Marine Bioprocess Research Center of Marine Bio 21 Project, funded by the Ministry of Maritime Affairs and Fisheries, Republic of Korea, for the support provided through the research grant, B-2005-02. We thank Dr. Sang-Oh Yoon (KAIST, Taejeon, Korea) for MMP-9 reporter vector.

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Authors and Affiliations

  1. Department of Chemistry, Pukyong National University, Busan, 608-737, Republic of Korea

    Quang Van Ta & Se-Kwon Kim

  2. Marine Bioprocess Research Center, Pukyong National University, Busan, 608-737, Republic of Korea

    Moon-Moo Kim & Se-Kwon Kim

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  1. Quang Van Ta
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  2. Moon-Moo Kim
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Correspondence to Se-Kwon Kim.

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Van Ta, Q., Kim, MM. & Kim, SK. Inhibitory Effect of Chitooligosaccharides on Matrix Metalloproteinase-9 in Human Fibrosarcoma Cells (HT1080). Mar Biotechnol 8, 593–599 (2006). https://doi.org/10.1007/s10126-006-6031-7

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  • DOI: https://doi.org/10.1007/s10126-006-6031-7

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