High-Yielding Enzymatic α-Glucosylation of Pyridoxine by Marine α-Glucosidase from Aplysia fasciata
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We recently succeeded in the identification and purification of an interesting marine exo-α-glucosidase (EC 126.96.36.199) from the anaspidean mollusc Aplysia fasciata. The enzyme was characterized by good transglycosylation activity toward different acceptors using maltose as donor. High-yielding enzymatic α-glycosylation of pyridoxine using this marine enzyme is reported here; the reaction has been optimized, reaching 80% molar yield of products (pyridoxine monoglucosides 24 g/l; pyridoxine isomaltoside 35 g/l). High selectivity toward the 5′ position is observed for both monoglucoside and disaccharide formation. This is the first report describing the enzymatic production of pyridoxine isomaltoside.
KeywordsAplysia fasciata biocatalysis α-glucosidase oligosaccharide synthesis marine organism transglycosylation
The authors wish to thank E. Pagnotta and R. D'Ambrosio for technical assistance, and S. Zambardino, D. Melck, and V. Mirra (NMR Service of Istituto di Chimica Biomolecolare, CNR, Pozzuoli, Italy). NMR spectra were obtained using a Bruker DPX-300 operating at 300 MHz, with a dual probe and/or on a Bruker Avance-400 operating at 400 MHz, using an inverse probe fitted with a gradient along the Z-axis. Dr. A. Tramice wishes to thank Centro Regionale di Competenza in Applicazioni Tecnologico-Industriali di Biomolecole e Biosistemi for a fellowship.
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