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Isolation of Marine Bacteria by In Situ Culture on Media-Supplemented Polyurethane Foam

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Abstract

Polyurethane foam (PUF) supplemented with various agar media was used in situ to trap marine bacteria and it consequently provided a substrate on which they could be cultivated while exposed to natural seawater in the coral reef area. The bacterial population on the PUF blocks was analyzed by denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR)-amplified 16S rDNA fragments. Changing the composition of the cultivation medium in the PUF blocks and selecting different sampling sites resulted in different bacteria being detected on the PUF blocks. For example, iron-utilizing (IU) bacteria, siderophore-producing (SP) bacteria, and petroleum-degrading (PD) bacteria were isolated from PUF blocks and it was discovered that IU and SP contained iron and PD contained hydrocarbon. This method opens up the possibility for isolating novel and useful marine bacteria.

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Acknowledgments

We thank Izumi Yamashima for technical assistance. We also thank Minoru Yasumoto for sampling. This work was part of The Industrial Science and Technology Project for Technology Development of Biological Resources in Bioconsortia, which is supported by the New Energy and Industrial Technology Development Organization of Japan, as part of the project on Constructing the Genetic Resource Library of Unidentified Microbes Based on Genome Information which is supported by the Ministry of Economy, Trade and Industry of Japan.

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Author notes

  1. Mina Yasumoto-Hirose

    Present address: JST Collaboration of Regional Entities for the Advancement of Technological Excellence in Okinawa, Okinawa Health Biotechnology Research Development Center, 12-75 Suzaki, Uruma, Okinawa, 904-2234, Japan

  2. Miyuki Nishijima

    Present address: NCIMB Japan Co. Ltd., 330 Nagasaki, Shimizu-ku, Shizuoka-shi, Shizuoka, 424-0065, Japan

  3. Metiek Kimie Ngirchechol

    Present address: Water Quality Laboratory, Palau Environmental Quality Protection Board, P.O. Box 100, Koror, Republic of Palau, 96940

Authors and Affiliations

  1. Marine Biotechnology Institute (MBI), 3-75-1 Heita, Kamaishi-shi, Iwate, 026-0001, Japan

    Mina Yasumoto-Hirose, Miyuki Nishijima, Metiek Kimie Ngirchechol, Kaneo Kanoh & Yoshikazu Shizuri

  2. SUNTORY Ltd., Institute for Advanced Technology, 1-1-1 Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka, 618-8503, Japan

    Wataru Miki

Authors
  1. Mina Yasumoto-Hirose
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  2. Miyuki Nishijima
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  4. Kaneo Kanoh
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  5. Yoshikazu Shizuri
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Correspondence to Mina Yasumoto-Hirose.

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Yasumoto-Hirose, M., Nishijima, M., Ngirchechol, M.K. et al. Isolation of Marine Bacteria by In Situ Culture on Media-Supplemented Polyurethane Foam. Mar Biotechnol 8, 227–237 (2006). https://doi.org/10.1007/s10126-005-5015-3

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  • DOI: https://doi.org/10.1007/s10126-005-5015-3

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