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Biological Activity of Sea Bass (Dicentrarchus labrax L.) Recombinant Interleukin-1β

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Abstract

Biological activities of a putative mature sea bass interleukin-1β peptide, produced as a recombinant protein (rIL-1β) in Escherichia coli, have been investigated. The rIL-1β contains a 6-histidine tag at the N-terminus, and protein purification has been achieved through this tag by affinity chromatography. Biological activities have been investigated both at the cellular and gene expression levels. In in vitro assays sea bass rIL-1β induced the proliferation of murine D10.G4.1 cells and increased yeast phagocytosis by sea bass head kidney leukocytes. The purified cytokine was also tested in a lymphocyte-activation factor assay, where it induced the proliferation of sea bass thymocytes. Finally, in an in vivo assay, rIL-1β administered intraperitoneally increased expression levels of the IL-1β gene and activated macrophages to produce a cyclooxygenase 2 homologue (COX-2) gene in the head kidney.

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Authors and Affiliations

  1. Dipartimento di Scienze Ambientali, Università della Tuscia, Viterbo, I-01100, Italy

    Francesco Buonocore, Maria Forlenza, Elisa Randelli, Stefania Benedetti, Paola Bossù, Sabrina Meloni, Massimo Mazzini & Giuseppe Scapigliati

  2. Scottish Fish Immunology Research Centre, University of Aberdeen, Aberdeen, AB24 2TZ, Scotland

    Christopher J. Secombes

Authors
  1. Francesco Buonocore
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  2. Maria Forlenza
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  3. Elisa Randelli
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  4. Stefania Benedetti
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  5. Paola Bossù
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  6. Sabrina Meloni
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  7. Christopher J. Secombes
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  8. Massimo Mazzini
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  9. Giuseppe Scapigliati
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Correspondence to Francesco Buonocore.

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Buonocore, F., Forlenza, M., Randelli, E. et al. Biological Activity of Sea Bass (Dicentrarchus labrax L.) Recombinant Interleukin-1β. Mar Biotechnol 7, 609–617 (2005). https://doi.org/10.1007/s10126-004-5131-5

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  • DOI: https://doi.org/10.1007/s10126-004-5131-5

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